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van Leeuwen PJ, Nieveen MC, van de Meent EJ, et al. Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach. Photosynth Res. 1991;28(3):149-53doi: 10.1007/BF00054128.
van Leeuwen, P. J., Nieveen, M. C., van de Meent, E. J., Dekker, J. P., & van Gorkom, H. J. (1991). Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach. Photosynthesis research, 28(3), 149-53. https://doi.org/10.1007/BF00054128
van Leeuwen, P J, et al. "Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach." Photosynthesis research vol. 28,3 (1991): 149-53. doi: https://doi.org/10.1007/BF00054128
van Leeuwen PJ, Nieveen MC, van de Meent EJ, Dekker JP, van Gorkom HJ. Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach. Photosynth Res. 1991 Jun;28(3):149-53. doi: 10.1007/BF00054128. PMID: 24414974.
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Photosynth Res. 1991 Jun;28(3):149-53. doi: 10.1007/BF00054128.

Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach.

Photosynthesis research

P J van Leeuwen, M C Nieveen, E J van de Meent, J P Dekker, H J van Gorkom

Affiliations

  1. Department of Biophysics, Huygens laboratory of the State University, P.O. Box 9504, 2300 RA, Leiden.

PMID: 24414974 DOI: 10.1007/BF00054128

Abstract

Pure and active oxygen-evolving PS II core particles containing 35 Chl per reaction center were isolated with 75% yield from spinach PS II membrane fragments by incubation with n-dodecyl-β-D-maltoside and a rapid one step anion-exchange separation. By Triton X-100 treatment on the column these particles could be converted with 55% yield to pure and active PS II reaction center particles, which contained 6 Chl per reaction center.

References

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