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Mandani P, Desai K, Highland H. Cytotoxic effects of benzene metabolites on human sperm function: an in vitro study. ISRN Toxicol. 2013;2013:397524doi: 10.1155/2013/397524.
Mandani, P., Desai, K., & Highland, H. (2013). Cytotoxic effects of benzene metabolites on human sperm function: an in vitro study. ISRN toxicology, 2013397524. https://doi.org/10.1155/2013/397524
Mandani, Priyanka, et al. "Cytotoxic effects of benzene metabolites on human sperm function: an in vitro study." ISRN toxicology vol. 2013 (2013): 397524. doi: https://doi.org/10.1155/2013/397524
Mandani P, Desai K, Highland H. Cytotoxic effects of benzene metabolites on human sperm function: an in vitro study. ISRN Toxicol. 2013 Dec 12;2013:397524. doi: 10.1155/2013/397524. eCollection 2013. PMID: 24416599; PMCID: PMC3874944.
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ISRN Toxicol. 2013 Dec 12;2013:397524. doi: 10.1155/2013/397524. eCollection 2013.

Cytotoxic effects of benzene metabolites on human sperm function: an in vitro study.

ISRN toxicology

Priyanka Mandani, Ketki Desai, Hyacinth Highland

Affiliations

  1. Department of Human Genetics, Biomedical Technology and Zoology, Gujarat University, Ahmedabad, Gujarat 380009, India.

PMID: 24416599 PMCID: PMC3874944 DOI: 10.1155/2013/397524

Abstract

In recent years, individuals are rampantly exposed to vapours of benzene, through paint, plastic, petroleum industries, fuel exhaust, and tobacco smoke. Hence the present investigation was directed towards determining the effect of benzene metabolites, namely, phenol-hydroquinone and catechol, on the motility, viability, and nuclear integrity of the human spermatozoa. From the results obtained it was clear that exposure to phenol-hydroquinone caused a significant decline in both, sperm motility and viability. Exposure to a phenol-hydroquinone (Phase I) microenvironment may therefore inhibit metabolically active enzymes, thus impeding ATP production, and in turn lowers sperm motility and viability. In addition, the present study also revealed that both metabolites of benzene caused significant denaturation of sperm nuclear DNA. Hence, exposure to phenol-hydroquinone in vitro could have resulted in generation of free radicals and altered membrane function, which is reflected by a decline in the motility, viability, and loss of sperm nuclear DNA integrity. In Phase II, the exposure of human sperm in vitro to varied concentrations of catechol caused only insignificant changes in sperm motility and viability as compared to those observed on exposure to phenol-hydroquinone. Hence, exposure to catechol appeared to have less toxic effects than those of phenol-hydroquinone.

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