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Jimbo R, Ivarsson M, Koskela A, et al. Protein adsorption to surface chemistry and crystal structure modification of titanium surfaces. J Oral Maxillofac Res. 2010;1(3):e3doi: 10.5037/jomr.2010.1303.
Jimbo, R., Ivarsson, M., Koskela, A., Sul, Y. T., & Johansson, C. B. (2010). Protein adsorption to surface chemistry and crystal structure modification of titanium surfaces. Journal of oral & maxillofacial research, 1(3), e3. https://doi.org/10.5037/jomr.2010.1303
Jimbo, Ryo, et al. "Protein adsorption to surface chemistry and crystal structure modification of titanium surfaces." Journal of oral & maxillofacial research vol. 1,3 (2010): e3. doi: https://doi.org/10.5037/jomr.2010.1303
Jimbo R, Ivarsson M, Koskela A, Sul YT, Johansson CB. Protein adsorption to surface chemistry and crystal structure modification of titanium surfaces. J Oral Maxillofac Res. 2010 Oct 01;1(3):e3. doi: 10.5037/jomr.2010.1303. eCollection 2010. PMID: 24421973; PMCID: PMC3886052.
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J Oral Maxillofac Res. 2010 Oct 01;1(3):e3. doi: 10.5037/jomr.2010.1303. eCollection 2010.

Protein adsorption to surface chemistry and crystal structure modification of titanium surfaces.

Journal of oral & maxillofacial research

Ryo Jimbo, Mikael Ivarsson, Anita Koskela, Young-Taeg Sul, Carina B Johansson

Affiliations

  1. Department of Prosthodontics, Faculty of Odontology, Malmö University Malmö Sweden. ; Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, Göteborg University Göteborg Sweden.
  2. Clinical Research Center, Örebro University Hosptial Örebro Sweden.
  3. Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, Göteborg University Göteborg Sweden. ; Institute for Clinical Dental Research, Korea University Seoul South Korea.
  4. Department of Clinical Medicine, School of Health and Medical Sciences, Örebro University Örebro Sweden.

PMID: 24421973 PMCID: PMC3886052 DOI: 10.5037/jomr.2010.1303

Abstract

OBJECTIVES: To observe the early adsorption of extracellular matrix and blood plasma proteins to magnesium-incorporated titanium oxide surfaces, which has shown superior bone response in animal models.

MATERIAL AND METHODS: Commercially pure titanium discs were blasted with titanium dioxide (TiO2) particles (control), and for the test group, TiO2 blasted discs were further processed with a micro-arc oxidation method (test). Surface morphology was investigated by scanning electron microscopy, surface topography by optic interferometry, characterization by X-ray photoelectron spectroscopy (XPS), and by X-ray diffraction (XRD) analysis. The adsorption of 3 different proteins (fibronectin, albumin, and collagen type I) was investigated by an immunoblotting technique.

RESULTS: The test surface showed a porous structure, whereas the control surface showed a typical TiO2 blasted structure. XPS data revealed magnesium-incorporation to the anodic oxide film of the surface. There was no difference in surface roughness between the control and test surfaces. For the protein adsorption test, the amount of albumin was significantly higher on the control surface whereas the amount of fibronectin was significantly higher on the test surface. Although there was no significant difference, the test surface had a tendency to adsorb more collagen type I.

CONCLUSIONS: The magnesium-incorporated anodized surface showed significantly higher fibronectin adsorption and lower albumin adsorption than the blasted surface. These results may be one of the reasons for the excellent bone response previously observed in animal studies.

Keywords: albumins; collagen type I.; fibronectins; immunoblotting; magnesium; titanium dioxide

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