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Costa JA, Leal-Pinto E, Henderson SC, et al. Use of a Pteridine Moiety to Track DNA Uptake in Cells. Pteridines. 2013;23(1):81-89doi: 10.1515/pteridines.2012.23.1.81.
Costa, J. A., Leal-Pinto, E., Henderson, S. C., Zabel, T., Hawkins, M. E., & Hanss, B. (2013). Use of a Pteridine Moiety to Track DNA Uptake in Cells. Pteridines, 23(1), 81-89. https://doi.org/10.1515/pteridines.2012.23.1.81
Costa, Justin A, et al. "Use of a Pteridine Moiety to Track DNA Uptake in Cells." Pteridines vol. 23,1 (2013): 81-89. doi: https://doi.org/10.1515/pteridines.2012.23.1.81
Costa JA, Leal-Pinto E, Henderson SC, Zabel T, Hawkins ME, Hanss B. Use of a Pteridine Moiety to Track DNA Uptake in Cells. Pteridines. 2013 Aug;23(1):81-89. doi: 10.1515/pteridines.2012.23.1.81. PMID: 24465092; PMCID: PMC3898856.
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Pteridines. 2013 Aug;23(1):81-89. doi: 10.1515/pteridines.2012.23.1.81.

Use of a Pteridine Moiety to Track DNA Uptake in Cells.

Pteridines

Justin A Costa, Edgar Leal-Pinto, Scott C Henderson, Troy Zabel, Mary E Hawkins, Basil Hanss

Affiliations

  1. Division of Nephrology, Department of Medicine, Mt. Sinai School of Medicine, New York, NY.
  2. Department of Physiology and Biophysics, Virginia Commonwealth University Medical Center, Richmond, VA.
  3. Department of Anatomy and Neurobiology, Virginia Commonwealth University Medical Center, Richmond, VA.
  4. South Denver Nephrology, Denver, CO.
  5. Laboratory of Receptor Biology and Gene Expression, NCI/NIH, Bethesda, MD.

PMID: 24465092 PMCID: PMC3898856 DOI: 10.1515/pteridines.2012.23.1.81

Abstract

Fluorescence labeled oligonucleotides have a long history of being used to monitor nucleic acid transport and uptake. However, it is not known if the fluorescent moiety itself physically limits the number of pathways that can be used by the cell due to steric, hydrophobic, or other chemical characteristics. Here, we report a method for comparing the uptake kinetics of oligonucleotides labeled either with the fluorescent pteridine, 3-methyl-8-(2-deoxy-β-D-ribofuranosyl) isoxanthopterin (3MI), or the common fluorophore 5-carboxyfluorescein (5-FAM). We use a multiphoton microscopic technique to monitor nucleic acid uptake LLC-PK

Keywords: DNA uptake monitoring; fluroescence label; methylisoxathopterin

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