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Rahmat AA, Dar FA, Choudhary IM. Protection of CCl4-Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis ferruginea (de Candolle). Pharmacognosy Res. 2014;6(1):19-28doi: 10.4103/0974-8490.122913.
Rahmat, A. A., Dar, F. A., & Choudhary, I. M. (2014). Protection of CCl4-Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis ferruginea (de Candolle). Pharmacognosy research, 6(1), 19-28. https://doi.org/10.4103/0974-8490.122913
Rahmat, Adisa A, et al. "Protection of CCl4-Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis ferruginea (de Candolle)." Pharmacognosy research vol. 6,1 (2014): 19-28. doi: https://doi.org/10.4103/0974-8490.122913
Rahmat AA, Dar FA, Choudhary IM. Protection of CCl4-Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis ferruginea (de Candolle). Pharmacognosy Res. 2014 Jan;6(1):19-28. doi: 10.4103/0974-8490.122913. PMID: 24497738; PMCID: PMC3897004.
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Pharmacognosy Res. 2014 Jan;6(1):19-28. doi: 10.4103/0974-8490.122913.

Protection of CCl4-Induced Liver and Kidney Damage by Phenolic Compounds in Leaf Extracts of Cnestis ferruginea (de Candolle).

Pharmacognosy research

Adisa A Rahmat, Farooq Ahsana Dar, Iqbal M Choudhary

Affiliations

  1. Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
  2. Pharmacology Unit, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, Pakistan.

PMID: 24497738 PMCID: PMC3897004 DOI: 10.4103/0974-8490.122913

Abstract

BACKGROUND: The chemoprevention of chemically-induced hepatotoxicity is a crucial means of minimizing susceptibility to hepatic carcinogenesis and plants remain a rich source of anti-hepatotoxicants with antioxidant properties.

OBJECTIVE: The protective role of defatted-methanol (MECF) and ethyl acetate fractions (EF), obtained from Leaves of Cnestis ferruginea in rats induced with carbon tetrachloride (CCl4) toxicity was investigated.

MATERIALS AND METHODS: Adult male Wistar rats were orally administered MECF or EF (125 - 500 mg/kg bwt/5mL) or silymarin (25 mg/kg bwt/5 mL) separately for three days before intervention with an intraperitoneal dose of CCl4. Biomarkers of liver and kidney toxicity as well as Ca(2+) regulation were evaluated.

RESULTS: Pre-treatment with MECF and EF significantly (P < 0.05) decreased the activities of serum alanine and aspartate aminotransferases, levels of urea, creatinine and cholesterol. A significantly (P < 0.05) enhanced Ca(2+) -ATPase activity and lowered levels of membrane cholesterol: Phospholipid ratio were observed in liver microsomes of pre-treated as compared to CCl4 -only treated rats. Rat liver superoxide dismutase activity was enhanced by 125 mg/kg and 250 mg/kg of EF and MECF, while decreases were observed at 500 mg/kg. MECF and EF, like silymarin, attenuated CCl4 -induced hepatotoxicity, microsomal membrane Ca(2+) -ATPase inactivation and renal dysfunction. Phytochemistry of MECF revealed the presence of anthraquinones, cardiac and flavone glycosides, tannins and trihydroxyl phenol.

CONCLUSION: These findings suggest that the mechanism of hepatoprotection elicited by MECF and EF, involve its antioxidative properties and regulation of Ca(2+) homeostasis.

Keywords: CCl4 -induced hepatotoxicity; Ca2+ -ATPase activity; Cnestis ferruginea; hepatoprotection; renal protection

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