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Prev Nutr Food Sci. 2012 Jun;17(2):136-40. doi: 10.3746/pnf.2012.17.2.136.

Bioactivity of Trifoliate Orange (Poncirus trifoliate) Seed Extracts.

Preventive nutrition and food science

Seong Yeong Kim, Kwang-Soon Shin

Affiliations

  1. Nutrition Education, Graduate School of Education, Kyonggi University, Gyeonggi 443-760, Korea.
  2. Department of Food Science and Biotechnology, Kyonggi University, Gyeonggi 443-760, Korea.

PMID: 24471075 PMCID: PMC3866750 DOI: 10.3746/pnf.2012.17.2.136

Abstract

Trifoliate orange seed extracts (TSEs) were made using either distilled water (TW), ethanol (TE), or n-hexane (TH), to measure total polyphenol contents, DPPH and ABTS radical scavenging activities, and anti-complementary activity. The results showed that the total polyphenol content showed higher value at TE (235.24 μg/mL, p<0.05) than those of TW (132.65 μg/mL) and TH (165.44 μg/mL) at 10 mg/mL and TE exerted the highest DPPH radical scavenging activity (61.77%, p<0.05), which occurred in the following order: TE>TW (56.87%)>TH (39.78%). The results of ABTS radical scavenging activity showed that TW (34.26%) and TE (31.81%) showed similar activities, which were higher than TH (12.74%, p<0.05). Anti-complementary activity of TE (61% at 500 μg/mL) showed a higher activity when compared with the positive control (60% at 1,000 μg/mL) polysaccharide-K (PSK), a known immuno-active polysaccharide from Coriolus versicolor. Consequently, among TSEs, TE is a byproduct from trifoliate orange and could be an important source of dietary polyphenolic antioxidant compounds and immunopotentiating activity, including complement activation.

Keywords: DPPH radical scavenging activity; anti-complementary activity; antioxidant activity; polyphenol; trifoliate orange seed extract

References

  1. Mol Carcinog. 2001 Apr;30(4):209-17 - PubMed
  2. Biol Pharm Bull. 2006 Feb;29(2):191-201 - PubMed
  3. Diabetes. 1991 Apr;40(4):405-12 - PubMed
  4. Carcinogenesis. 1998 Jan;19(1):207-12 - PubMed
  5. J Agric Food Chem. 2003 Dec 3;51(25):7292-5 - PubMed
  6. Free Radic Biol Med. 1999 May;26(9-10):1231-7 - PubMed
  7. Clin Exp Pharmacol Physiol. 2006 Jan-Feb;33(1-2):146-51 - PubMed
  8. J Agric Food Chem. 2000 Oct;48(10):4581-9 - PubMed
  9. Toxicology. 1997 Sep 26;122(1-2):61-72 - PubMed
  10. Arch Biochem Biophys. 2002 Jan 15;397(2):377-83 - PubMed
  11. Food Chem Toxicol. 1994 Jul;32(7):671-83 - PubMed
  12. Mutat Res. 2003 Feb-Mar;523-524:9-20 - PubMed
  13. Molecules. 2010 Oct 21;15(10):7313-52 - PubMed
  14. Free Radic Biol Med. 2000 Mar 1;28(5):831-4 - PubMed
  15. J Enzyme Inhib Med Chem. 2007 Dec;22(6):685-95 - PubMed

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