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Nikbin VS, Shahcheraghi F, Lotfi MN, et al. Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran. Iran J Microbiol. 2013;5(3):209-14
Nikbin, V. S., Shahcheraghi, F., Lotfi, M. N., Zahraei, S. M., & Parzadeh, M. (2013). Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran. Iranian journal of microbiology, 5(3), 209-14.
Nikbin, Vajiheh Sadat, et al. "Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran." Iranian journal of microbiology vol. 5,3 (2013): 209-14.
Nikbin VS, Shahcheraghi F, Lotfi MN, Zahraei SM, Parzadeh M. Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran. Iran J Microbiol. 2013 Sep;5(3):209-14. PMID: 24475325; PMCID: PMC3895556.
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Iran J Microbiol. 2013 Sep;5(3):209-14.

Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran.

Iranian journal of microbiology

Vajiheh Sadat Nikbin, Fereshteh Shahcheraghi, Masoumeh Nakhost Lotfi, Seyyed Mohsen Zahraei, Masoumeh Parzadeh

Affiliations

  1. Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran.
  2. Center for Disease Control, Ministry of Health and Medical Education, Tehran, Iran.

PMID: 24475325 PMCID: PMC3895556

Abstract

BACKGROUND AND OBJECTIVE: Due to contagiousness of pertussis, a rapid and sensitive method for diagnosis is required to initiate the treatment and interrupt its transmission.

MATERIALS AND METHODS: To detect B. pertussis strains, we used two real-time PCR targeting IS481 and BP283 sequences and compared factors influencing culture and real-time PCR results.

RESULTS: Totally, 779 specimens were collected from patients among which 11 (1.4%) were culture positive. Using IS481 and BP283 primers, 122 (15.6%) and 100 (12.8%) were diagnosed as infected specimens respectively. There were significant relationships between the real-time PCR method for diagnosis of B. pertussis and age, sex and vaccination of patients before sampling.

CONCLUSION: The real-time PCR is superior and much more sensitive than culture for diagnosis of B. pertussis. However, the sensitivity was improved when both IS481 and BP283 were used. Correct sampling and transportation of specimen also improved the detection rate in our research.

Keywords: BP283; Bordetella pertussis; IS481; real-time PCR

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