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Kuboyama N, Abiko Y. Reduction of monocyte chemoattractant protein-1 expression in rheumatoid arthritis rat joints with light-emitting diode phototherapy. Laser Ther. 2012;21(3):177-81doi: 10.5978/islsm.12-OR-08.
Kuboyama, N., & Abiko, Y. (2012). Reduction of monocyte chemoattractant protein-1 expression in rheumatoid arthritis rat joints with light-emitting diode phototherapy. Laser therapy, 21(3), 177-81. https://doi.org/10.5978/islsm.12-OR-08
Kuboyama, Noboru, and Abiko, Yoshimitsu. "Reduction of monocyte chemoattractant protein-1 expression in rheumatoid arthritis rat joints with light-emitting diode phototherapy." Laser therapy vol. 21,3 (2012): 177-81. doi: https://doi.org/10.5978/islsm.12-OR-08
Kuboyama N, Abiko Y. Reduction of monocyte chemoattractant protein-1 expression in rheumatoid arthritis rat joints with light-emitting diode phototherapy. Laser Ther. 2012 Sep 30;21(3):177-81. doi: 10.5978/islsm.12-OR-08. Epub 2012 Aug 11. PMID: 24511187; PMCID: PMC3882353.
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Laser Ther. 2012 Sep 30;21(3):177-81. doi: 10.5978/islsm.12-OR-08. Epub 2012 Aug 11.

Reduction of monocyte chemoattractant protein-1 expression in rheumatoid arthritis rat joints with light-emitting diode phototherapy.

Laser therapy

Noboru Kuboyama, Yoshimitsu Abiko

Affiliations

  1. Department of Molecular Pharmacology, Nihon University School of Dentistry at Matsudo, Chiba 271, Japan.
  2. Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, Chiba 271, Japan.

PMID: 24511187 PMCID: PMC3882353 DOI: 10.5978/islsm.12-OR-08

Abstract

BACKGROUND: Rheumatoid arthritis (RA) is a systemic autoimmune disorder that involves inflammation and pain of the joints. Light-Emitting Diode (LED) irradiation is being evaluated for treating RA; however, the mechanism is unclear. Monocyte chemotaxis protein (MCP)-1 is a key chemokine in the inflammatory status of RA, and MCP-1 levels in plasma are described as a marker for joint inflammation in RA.

AIM: To understand the mechanism of the anti-inflammatory effect of LED irradiation on RA, the expression of MCP-1 was examined in the knee joints of collagen-induced arthritis (CIA) rats.

MATERIALS AND METHODS: The rats were immunized with type II collagen and CIA development was confirmed. CIA rat joints were irradiated with LED energy (3 sessions/week, 2 weeks. 840 nm, 2 J/cm(2)). Total RNA was isolated from the rat knee joint tissues and the MCP-1 mRNA levels were monitored with the reverse transcription polymerase chain reaction (RT-PCR) technique and real-time PCR. MCP-1 production in the rat knee joints was analyzed immunohistochemically.

RESULTS: RT-PCR analysis demonstrated that MCP-1 mRNA levels had increased in CIA animals when compared to controls, and LED irradiation significant reduced the gene expression in CIA rats. Real-time PCR analysis confirmed a significant reduction in MCP-1 gene expression. The immunohistochemical analysis demonstrated strong MCP-1 staining in CIA rat joint synovial membrane tissue, and LED irradiation significantly reduced the staining.

DISCUSSION: Since MCP-1 has been identified as an important chemokine in the pathogenesis of RA, the reduction of MCP-1 expression would appear to be one of the mechanisms in the reduction of inflammation by LED irradiation.

CONCLUSION: LED irradiation reduced RA-related inflammation through the reduction of MCP-1 gene expression in CIA rat knee joint synovial tissue.

Keywords: LED; MCP-1; Rheumatoid arthritis; rat joint

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