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Kopylov AT, Kuznetsova KG, Mikhailova OM, et al. Development of mass spectrometry selected reaction monitoring method for quantitation and pharmacokinetic study of stepharine in rabbit plasma. Adv Pharmacol Sci. 2014;2014:961850doi: 10.1155/2014/961850.
Kopylov, A. T., Kuznetsova, K. G., Mikhailova, O. M., Moshkin, A. G., Turkin, V. V., & Alimov, A. A. (2014). Development of mass spectrometry selected reaction monitoring method for quantitation and pharmacokinetic study of stepharine in rabbit plasma. Advances in pharmacological sciences, 2014961850. https://doi.org/10.1155/2014/961850
Kopylov, Arthur T, et al. "Development of mass spectrometry selected reaction monitoring method for quantitation and pharmacokinetic study of stepharine in rabbit plasma." Advances in pharmacological sciences vol. 2014 (2014): 961850. doi: https://doi.org/10.1155/2014/961850
Kopylov AT, Kuznetsova KG, Mikhailova OM, Moshkin AG, Turkin VV, Alimov AA. Development of mass spectrometry selected reaction monitoring method for quantitation and pharmacokinetic study of stepharine in rabbit plasma. Adv Pharmacol Sci. 2014;2014:961850. doi: 10.1155/2014/961850. Epub 2014 Feb 20. PMID: 24696679; PMCID: PMC3950497.
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Adv Pharmacol Sci. 2014;2014:961850. doi: 10.1155/2014/961850. Epub 2014 Feb 20.

Development of mass spectrometry selected reaction monitoring method for quantitation and pharmacokinetic study of stepharine in rabbit plasma.

Advances in pharmacological sciences

Arthur T Kopylov, Ksenia G Kuznetsova, Olga M Mikhailova, Andrey G Moshkin, Vladimir V Turkin, Andrei A Alimov

Affiliations

  1. Institute of Biomedical Chemistry, 10 Pogodinskaya Street, Moscow 119121, Russia.
  2. Institute of Applied Biochemistry JSC "Biochimmash," 4 KlaraTsetkin Street, Moscow 127299, Russia.

PMID: 24696679 PMCID: PMC3950497 DOI: 10.1155/2014/961850

Abstract

Highly sensitive liquid chromatography mass spectrometry method on triple quadrupole (QQQ) mass spectrometer was successfully applied for pharmacokinetic study of stepharine in rabbit plasma. Specific ion transitions of stepharine protonated precursor ion were selected and recorded in the certain retention time employing dynamic selected reaction monitoring mode. The developed method facilitated quantitative measurements of stepharine in plasma samples in linear range of five orders of magnitude with high accuracy and low standard deviation coefficient and pharmacokinetics parameters were calculated. The apparent volume of stepharine distribution (estimated as ratio of clearance to elimination rate constant, data not shown) allows us to assume that stepharine was extensively distributed throughout the body.

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