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Bellstedt P, Ihle Y, Wiedemann C, et al. Sequential acquisition of multi-dimensional heteronuclear chemical shift correlation spectra with ¹H detection. Sci Rep. 2014;4:4490doi: 10.1038/srep04490.
Bellstedt, P., Ihle, Y., Wiedemann, C., Kirschstein, A., Herbst, C., Görlach, M., & Ramachandran, R. (2014). Sequential acquisition of multi-dimensional heteronuclear chemical shift correlation spectra with ¹H detection. Scientific reports, 44490. https://doi.org/10.1038/srep04490
Bellstedt, Peter, et al. "Sequential acquisition of multi-dimensional heteronuclear chemical shift correlation spectra with ¹H detection." Scientific reports vol. 4 (2014): 4490. doi: https://doi.org/10.1038/srep04490
Bellstedt P, Ihle Y, Wiedemann C, Kirschstein A, Herbst C, Görlach M, Ramachandran R. Sequential acquisition of multi-dimensional heteronuclear chemical shift correlation spectra with ¹H detection. Sci Rep. 2014 Mar 27;4:4490. doi: 10.1038/srep04490. PMID: 24671105; PMCID: PMC3967198.
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Sci Rep. 2014 Mar 27;4:4490. doi: 10.1038/srep04490.

Sequential acquisition of multi-dimensional heteronuclear chemical shift correlation spectra with ¹H detection.

Scientific reports

Peter Bellstedt, Yvonne Ihle, Christoph Wiedemann, Anika Kirschstein, Christian Herbst, Matthias Görlach, Ramadurai Ramachandran

Affiliations

  1. Leibniz Institute for Age Research - Fritz Lipmann Institute, Department Biomolecular NMR Spectroscopy, Beutenbergstraße 11, 07745 Jena, Germany.
  2. 1] Leibniz Institute for Age Research - Fritz Lipmann Institute, Department Biomolecular NMR Spectroscopy, Beutenbergstraße 11, 07745 Jena, Germany [2].
  3. Ubon Ratchathani University, Department of Physics, 34190 Ubon Ratchathani, Thailand.

PMID: 24671105 PMCID: PMC3967198 DOI: 10.1038/srep04490

Abstract

RF pulse schemes for the simultaneous acquisition of heteronuclear multi-dimensional chemical shift correlation spectra, such as {HA(CA)NH & HA(CACO)NH}, {HA(CA)NH & H(N)CAHA} and {H(N)CAHA & H(CC)NH}, that are commonly employed in the study of moderately-sized protein molecules, have been implemented using dual sequential (1)H acquisitions in the direct dimension. Such an approach is not only beneficial in terms of the reduction of experimental time as compared to data collection via two separate experiments but also facilitates the unambiguous sequential linking of the backbone amino acid residues. The potential of sequential (1)H data acquisition procedure in the study of RNA is also demonstrated here.

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