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Kheradmand F, Nourmohammadi I, Ahmadi-Faghih MA, et al. Zinc and low-dose of cadmium protect sertoli cells against toxic-dose of cadmium: The role of metallothionein. Iran J Reprod Med. 2013;11(6):487-94
Kheradmand, F., Nourmohammadi, I., Ahmadi-Faghih, M. A., Firoozrai, M., & Modarressi, M. H. (2013). Zinc and low-dose of cadmium protect sertoli cells against toxic-dose of cadmium: The role of metallothionein. Iranian journal of reproductive medicine, 11(6), 487-94.
Kheradmand, Fatemeh, et al. "Zinc and low-dose of cadmium protect sertoli cells against toxic-dose of cadmium: The role of metallothionein." Iranian journal of reproductive medicine vol. 11,6 (2013): 487-94.
Kheradmand F, Nourmohammadi I, Ahmadi-Faghih MA, Firoozrai M, Modarressi MH. Zinc and low-dose of cadmium protect sertoli cells against toxic-dose of cadmium: The role of metallothionein. Iran J Reprod Med. 2013 Jun;11(6):487-94. PMID: 24639783; PMCID: PMC3941315.
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Iran J Reprod Med. 2013 Jun;11(6):487-94.

Zinc and low-dose of cadmium protect sertoli cells against toxic-dose of cadmium: The role of metallothionein.

Iranian journal of reproductive medicine

Fatemeh Kheradmand, Issa Nourmohammadi, Mohamad Amin Ahmadi-Faghih, Mohsen Firoozrai, Mohammad Hossein Modarressi

Affiliations

  1. Department of Biochemistry, Faculty of Medicine and Center for Cellular and Molecular Research, Urmia University of Medical Sciences, Urmia, Iran. ; Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  2. Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
  3. Department of Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

PMID: 24639783 PMCID: PMC3941315

Abstract

BACKGROUND: The impact of cadmium (Cd) on male infertility may be related to the interaction with metal-binding proteins known as metallothioneins (Mts). Trace elements like zinc (Zn) have protective effects on testicular damage induced by Cd.

OBJECTIVE: We determined the effect of Zn and low-dose Cd pre-treatment on the expression of Mt1 and Mt2 genes on testicular Sertoli cells.

MATERIALS AND METHODS: The cultured TM4 mouse sertoli cells were treated with 50 μM ZnSO4 (Zn pre-treated group; ZnPG), 2 μM CdCl2 (Cd pre-treated group; CdPG), or distilled water (DW pre-treated group; DWPG). After 18 hour, all of these groups were exposed to 100 μM CdCl2 for different periods of time (1, 2, 3, and 6 hours). There was also a control group for all three groups, which was treated only with distilled water (without Cd or Zn pre-treatment). Cellular viability, Zn and Cd concentrations and gene expression were assessed by MTT, atomic absorption spectrometry and real time PCR methods, respectively.

RESULTS: The expression of Mt1 and Mt2 genes in ZnPG, CdPG, and DWPG was greater than the control group (p=0.02 and p=0.01, respectively). Cd concentrations in CdPG and DWPG were greater than the control group (p=0.00). Expression of both genes in ZnPG and CdPG increased after 3 hours of treatment and Cd concentration decreased simultaneously, which was more obvious in ZnPG.

CONCLUSION: Zn and short term low-dose Cd pre-treatment might reduce the adverse effects of Cd by increasing expression of Mts genes in Sertoli cells. The protective effect of Zn was stronger than Cd.

Keywords: Cadmium; Gene expression; Metallothionein; Sertoli cells; Zinc

References

  1. DNA Cell Biol. 2009 May;28(5):241-8 - PubMed
  2. Biometals. 2004 Oct;17(5):555-8 - PubMed
  3. Toxicology. 1997 Mar 28;118(2-3):85-92 - PubMed
  4. Reprod Toxicol. 2003 Mar-Apr;17(2):219-27 - PubMed
  5. J Cell Biochem. 1994 Aug;55(4):486-95 - PubMed
  6. Wei Sheng Yan Jiu. 2000 May 30;29(3):135-7 - PubMed
  7. Ind Health. 2009 Oct;47(5):487-94 - PubMed
  8. FEBS Lett. 1974 Feb 15;39(2):229-34 - PubMed
  9. Toxicol Appl Pharmacol. 2001 Oct 1;176(1):1-9 - PubMed
  10. Biol Trace Elem Res. 2012 Jun;146(3):410-9 - PubMed
  11. Biometals. 2004 Dec;17(6):615-24 - PubMed
  12. Bull Environ Contam Toxicol. 2008 Dec;81(6):521-4 - PubMed
  13. World J Gastroenterol. 2003 Jul;9(7):1554-8 - PubMed
  14. Fundam Appl Toxicol. 1990 Nov;15(4):641-50 - PubMed
  15. Reprod Biol. 2006 Mar;6(1):17-30 - PubMed
  16. Mol Med. 2009 Jul-Aug;15(7-8):248-62 - PubMed
  17. J Environ Biol. 2004 Jul;25(3):251-8 - PubMed
  18. Toxicol Appl Pharmacol. 2009 Aug 1;238(3):240-9 - PubMed
  19. Cell Biol Toxicol. 2004 Jul;20(4):241-51 - PubMed
  20. Toxicol Appl Pharmacol. 2009 Aug 1;238(3):215-20 - PubMed
  21. Fundam Appl Toxicol. 1995 Jun;26(1):99-106 - PubMed
  22. Toxicol Appl Pharmacol. 1976 Sep;37(3):403-14 - PubMed
  23. Biol Trace Elem Res. 1991 Dec;31(3):209-14 - PubMed
  24. J Carcinog. 2006 Jun 02;5:17 - PubMed
  25. Reprod Biol Endocrinol. 2010 Aug 16;8:97 - PubMed
  26. J Inorg Biochem. 2000 Apr;79(1-4):241-4 - PubMed
  27. Toxicol Appl Pharmacol. 1983 Sep 15;70(2):195-203 - PubMed
  28. Congenit Anom (Kyoto). 2003 Dec;43(4):271-9 - PubMed
  29. J Toxicol Environ Health. 1994 Feb;41(2):233-45 - PubMed
  30. Exp Toxicol Pathol. 2010 May;62(3):281-8 - PubMed
  31. J Reprod Dev. 2008 Apr;54(2):129-34 - PubMed
  32. Hum Exp Toxicol. 1994 Jan;13(1):65-7 - PubMed
  33. Biometals. 2008 Feb;21(1):71-81 - PubMed
  34. Toxicology. 2001 Jun 21;163(2-3):93-100 - PubMed
  35. Biol Trace Elem Res. 2005 Jun;104(3):223-32 - PubMed
  36. Arch Toxicol. 2009 Jul;83(7):647-52 - PubMed
  37. Wei Sheng Yan Jiu. 2000 Mar 30;29(2):78-9 - PubMed
  38. Annu Rev Pharmacol Toxicol. 1999;39:267-94 - PubMed
  39. Wei Sheng Yan Jiu. 2003 Nov;32(6):618-9 - PubMed
  40. Biometals. 2004 Oct;17(5):571-2 - PubMed
  41. Arch Androl. 1999 Sep-Oct;43(2):135-40 - PubMed
  42. Exp Mol Pathol. 1976 Oct;25(2):163-71 - PubMed
  43. J Reprod Fertil. 1982 Jul;65(2):489-95 - PubMed
  44. J Trace Elem Med Biol. 2010 Oct;24(4):277-82 - PubMed
  45. Biomed Environ Sci. 2000 Mar;13(1):19-25 - PubMed
  46. Reprod Toxicol. 2008 Apr;25(3):304-15 - PubMed

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