J Glob Infect Dis. 2013 Oct;5(4):131-7. doi: 10.4103/0974-777X.122001.
The Plasmodium falciparum Antigen MB2 Induces Interferon-γ and Interleukin-10 Responses in Adults in Malaria Endemic Areas of Western Kenya.
Journal of global infectious diseases
Lyticia A Ochola, Gideon M Ng'wena, Gregory S Noland, Bartholomew N Ondigo, George Ayodo, Chandy C John
Affiliations
Affiliations
- Department of Biomedical Sciences and Technology, School of Public Health and Community Development, Maseno University, Maseno ; Kenya Medical Research Institute, University of Minnesota Malaria Program, Kisumu, Kenya.
- Department of Medical Physiology, School of Medicine, Maseno University, Maseno, Kenya.
- Department of Pediatrics, University of Minnesota, Minneapolis, USA.
- Department of Biomedical Sciences and Technology, School of Public Health and Community Development, Maseno University, Maseno.
- Kenya Medical Research Institute, University of Minnesota Malaria Program, Kisumu, Kenya.
PMID: 24672173
PMCID: PMC3958981 DOI: 10.4103/0974-777X.122001
Abstract
BACKGROUND: MB2 is a novel Plasmodium falciparum antigen of unknown function expressed in pre-erythrocytic and blood stages of infection in the human host. Interferon-gamma (IFN-γ) and interleukin (IL)-10 responses to other P. falciparum antigens have been associated with protection from clinical malaria, but these responses have not been studied for MB2. The present study was undertaken to characterize IFN-γ and IL-10 responses to P. falciparum MB2 antigen in adults living in areas of differing malaria transmission in Western Kenya.
MATERIALS AND METHODS: Cytokine responses to two 9-mer MB2 peptides predicted to be human leukocyte antigen (HLA) class I restricted T-cell epitopes were measured by enzyme-linked immunosorbent assay (ELISA) (IFN-γ and IL-10) and enzyme-linked immunosorbent spot (ELISPOT) (IFN-γ) in adults (n = 228) in areas of unstable and stable malaria transmission. HLA class I restriction of responses was assessed in a sub-group of the study population.
RESULTS: IFN-γ and IL-10 responses to MB2 peptides by ELISA were observed in both sites with no significant difference in prevalence (IFN-γ, unstable transmission area, 18.8%, stable transmission area, 27.5%, P = 0.33; IL-10, unstable transmission area, 22.5%, stable transmission area, 25.0%, P = 0.78). Prevalence of IFN-γ responses by ELISPOT was also similar in both areas (unstable, 10.8%, stable, 10.9%, P = 0.98). Neither IFN-γ nor IL-10 responses showed evidence of HLA class I restriction.
CONCLUSIONS: MB2 induces IFN-γ and IL-10 responses in adults living in both stable and unstable malaria transmission areas. Future studies should assess if these responses are associated with protection from clinical malaria.
Keywords: Enzyme-linked immunosorbent assay; Enzyme-linked immunosorbent spot; Human leukocyte antigen; Interferon-gamma; Interleukin-10; MB2; Malaria; Plasmodium falciparum
References
- Infect Immun. 2004 Sep;72(9):5135-42 - PubMed
- Infect Immun. 1999 Jul;67(7):3424-9 - PubMed
- Malar J. 2003 Nov 5;2(1):37 - PubMed
- PLoS One. 2009 Jul 31;4(7):e6465 - PubMed
- Southeast Asian J Trop Med Public Health. 2009 Jan;40(1):10-7 - PubMed
- Trans R Soc Trop Med Hyg. 2005 Oct;99(10):780-6 - PubMed
- J Infect Dis. 2009 Aug 1;200(3):329-36 - PubMed
- Am J Trop Med Hyg. 2009 Sep;81(3):489-95 - PubMed
- Annu Rev Immunol. 2001;19:683-765 - PubMed
- J Infect Dis. 1999 Apr;179(4):980-8 - PubMed
- Infect Immun. 2005 Sep;73(9):5799-808 - PubMed
- J Med Entomol. 1990 Jul;27(4):570-7 - PubMed
- Am J Trop Med Hyg. 2006 Apr;74(4):585-90 - PubMed
- Clin Diagn Lab Immunol. 2002 Mar;9(2):348-51 - PubMed
- Malar J. 2009 Oct 23;8:235 - PubMed
- J Infect Dis. 1999 Jan;179(1):279-82 - PubMed
- J Immunol. 1999 Jul 15;163(2):884-92 - PubMed
- Nat Med. 2004 Apr;10(4):406-10 - PubMed
- Lancet. 1998 Jun 13;351(9118):1768-72 - PubMed
- Med Microbiol Immunol. 2001 Apr;189(3):115-26 - PubMed
- Clin Exp Immunol. 2000 Oct;122(1):94-100 - PubMed
- PLoS One. 2008 Apr 30;3(4):e2027 - PubMed
- J Biol Chem. 2001 Jul 13;276(28):26724-31 - PubMed
- Infect Immun. 1997 Dec;65(12):5082-7 - PubMed
- Am J Trop Med Hyg. 1999 Sep;61(3):488-94 - PubMed
- Infect Immun. 2000 Sep;68(9):5198-204 - PubMed
- N Engl J Med. 2011 Nov 17;365(20):1863-75 - PubMed
- Infect Immun. 1990 Sep;58(9):2989-94 - PubMed
- Tissue Antigens. 2004 Apr;63(4):293-325 - PubMed
- Infect Immun. 2009 Oct;77(10):4502-9 - PubMed
- J Immunol. 2008 Jan 1;180(1):444-53 - PubMed
- J Immunol. 2003 Dec 15;171(12):6961-7 - PubMed
- PLoS One. 2011;6(10):e25786 - PubMed
- Am J Trop Med Hyg. 2003 Apr;68(4):421-30 - PubMed
- Mol Biol Evol. 1999 May;16(5):627-33 - PubMed
- J Infect Dis. 1998 Aug;178(2):520-5 - PubMed
- Am J Trop Med Hyg. 2012 Nov;87(5):806-12 - PubMed
Publication Types
Grant support