Display options
Cite
Wang S, Ye SD, Sun WJ, et al. Pioglitazone Inhibits the Expressions of p22(phox) and p47(phox) in Rat Mesangial Cells In Vitro. ISRN Endocrinol. 2014;2014:601352doi: 10.1155/2014/601352.
Wang, S., Ye, S. D., Sun, W. J., & Hu, Y. Y. (2014). Pioglitazone Inhibits the Expressions of p22(phox) and p47(phox) in Rat Mesangial Cells In Vitro. ISRN endocrinology, 2014601352. https://doi.org/10.1155/2014/601352
Wang, Shan, et al. "Pioglitazone Inhibits the Expressions of p22(phox) and p47(phox) in Rat Mesangial Cells In Vitro." ISRN endocrinology vol. 2014 (2014): 601352. doi: https://doi.org/10.1155/2014/601352
Wang S, Ye SD, Sun WJ, Hu YY. Pioglitazone Inhibits the Expressions of p22(phox) and p47(phox) in Rat Mesangial Cells In Vitro. ISRN Endocrinol. 2014 Feb 03;2014:601352. doi: 10.1155/2014/601352. eCollection 2014. PMID: 24639901; PMCID: PMC3929998.
Copy Download .nbib Format: NLM
Share it on

ISRN Endocrinol. 2014 Feb 03;2014:601352. doi: 10.1155/2014/601352. eCollection 2014.

Pioglitazone Inhibits the Expressions of p22(phox) and p47(phox) in Rat Mesangial Cells In Vitro.

ISRN endocrinology

Shan Wang, Shan-Dong Ye, Wen-Jia Sun, Yuan-Yuan Hu

Affiliations

  1. Department of Endocrinology, Anhui Provincial Hospital Affiliated to Anhui Medical University, No. 17 Lujiang Road, Hefei 230001, China.

PMID: 24639901 PMCID: PMC3929998 DOI: 10.1155/2014/601352

Abstract

Aim. The purpose of this study was to investigate the effects of pioglitazone on oxidative stress and the expressions of p22(phox) and p47(phox), subunits of NADPH oxidase, in mesangial cells (MCs). Method. Rat mesangial cells were cultured and randomly divided into normal glucose (NG) group, high glucose (HG) group, and pioglitazone group. After 48 h exposure, the supernatants and cells were collected. The expressions of p22(phox) and p47(phox) in MCs were detected by RT-PCR and western blot. The levels of intracellular ROS were determined by flow cytometry. Coloimetry method was used to detect malondialdehyde (MDA) concentrations and superoxide dismutase (SOD) activities. Results. Compared with the NG group, the expression levels of p22(phox), p47(phox) and ROS significantly increased, the activity of SOD decreased in HG group, while the concentration of MDA greatly increased (P < 0.01). Pioglitazone significantly suppressed HG-induced p22(phox) and p47(phox) expressions and oxidative stress. The protein and gene expressions of p22(phox) and p47(phox) were markedly reduced after pioglitazone treatment, so did the ROS generation. The activities of SOD in MCs increased, while the concentrations of MDA in the supernatant decreased greatly by pioglitazone. Conclusions. Pioglitazone can inhibit HG-induced oxidative stress in MCs through suppressing p22(phox) and p47(phox) expressions.

References

  1. Environ Toxicol Pharmacol. 2009 Jul;28(1):30-6 - PubMed
  2. Exp Biol Med (Maywood). 2008 Jan;233(1):4-11 - PubMed
  3. Int J Cardiol. 2012 Jun 14;157(3):413-5 - PubMed
  4. Postgrad Med. 2012 Nov;124(6):90-7 - PubMed
  5. Clin Endocrinol (Oxf). 2010 Dec;73(6):739-43 - PubMed
  6. Toxicol In Vitro. 2011 Jun;25(4):839-47 - PubMed
  7. Diabetes Res Clin Pract. 2010 Mar;87(3):313-21 - PubMed
  8. Nat Rev Endocrinol. 2011 Mar;7(3):176-84 - PubMed
  9. Nihon Jinzo Gakkai Shi. 2011;53(7):1016-20 - PubMed
  10. J Am Soc Nephrol. 2009 Nov;20(11):2380-8 - PubMed
  11. Lab Invest. 2008 Dec;88(12):1316-28 - PubMed
  12. Cell Commun Signal. 2012 Nov 15;10(1):33 - PubMed
  13. Ann Indian Acad Neurol. 2012 Oct;15(4):281-6 - PubMed
  14. J Appl Toxicol. 2014 Jan;34(1):25-32 - PubMed
  15. Nephrol Dial Transplant. 2009 Aug;24(8):2384-91 - PubMed
  16. Diabetes. 2008 Jun;57(6):1439-45 - PubMed
  17. Oxid Med Cell Longev. 2012;2012:856085 - PubMed
  18. Pharmacol Rev. 2011 Mar;63(1):218-42 - PubMed
  19. Pharmacol Rep. 2012;64(5):1223-33 - PubMed
  20. Curr Diabetes Rev. 2011 Sep;7(5):313-24 - PubMed
  21. Kidney Blood Press Res. 2011;34(1):20-33 - PubMed
  22. Int J Mol Sci. 2013 Feb 05;14(2):3265-84 - PubMed

Publication Types