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Psomopoulos FE, Siarkou VI, Papanikolaou N, et al. The chlamydiales pangenome revisited: structural stability and functional coherence. Genes (Basel). 2012;3(2):291-319doi: 10.3390/genes3020291.
Psomopoulos, F. E., Siarkou, V. I., Papanikolaou, N., Iliopoulos, I., Tsaftaris, A. S., Promponas, V. J., & Ouzounis, C. A. (2012). The chlamydiales pangenome revisited: structural stability and functional coherence. Genes, 3(2), 291-319. https://doi.org/10.3390/genes3020291
Psomopoulos, Fotis E, et al. "The chlamydiales pangenome revisited: structural stability and functional coherence." Genes vol. 3,2 (2012): 291-319. doi: https://doi.org/10.3390/genes3020291
Psomopoulos FE, Siarkou VI, Papanikolaou N, Iliopoulos I, Tsaftaris AS, Promponas VJ, Ouzounis CA. The chlamydiales pangenome revisited: structural stability and functional coherence. Genes (Basel). 2012 May 16;3(2):291-319. doi: 10.3390/genes3020291. PMID: 24704919; PMCID: PMC3899948.
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Genes (Basel). 2012 May 16;3(2):291-319. doi: 10.3390/genes3020291.

The chlamydiales pangenome revisited: structural stability and functional coherence.

Genes

Fotis E Psomopoulos, Victoria I Siarkou, Nikolas Papanikolaou, Ioannis Iliopoulos, Athanasios S Tsaftaris, Vasilis J Promponas, Christos A Ouzounis

Affiliations

  1. Institute of Agrobiotechnology, Centre for Research & Technology Hellas (CERTH), Thessaloniki GR-57001, Greece. [email protected].
  2. Laboratory of Microbiology & Infectious Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki GR-54124, Greece. [email protected].
  3. Division of Medical Sciences, University of Crete Medical School, Heraklion GR-71110, Greece. [email protected].
  4. Division of Medical Sciences, University of Crete Medical School, Heraklion GR-71110, Greece. [email protected].
  5. Institute of Agrobiotechnology, Centre for Research & Technology Hellas (CERTH), Thessaloniki GR-57001, Greece. [email protected].
  6. Bioinformatics Research Laboratory, Department of Biological Sciences, University of Cyprus, P.O. Box 20537, Nicosia CY-1678, Cyprus. [email protected].
  7. Institute of Agrobiotechnology, Centre for Research & Technology Hellas (CERTH), Thessaloniki GR-57001, Greece. [email protected].

PMID: 24704919 PMCID: PMC3899948 DOI: 10.3390/genes3020291

Abstract

The entire publicly available set of 37 genome sequences from the bacterial order Chlamydiales has been subjected to comparative analysis in order to reveal the salient features of this pangenome and its evolutionary history. Over 2,000 protein families are detected across multiple species, with a distribution consistent to other studied pangenomes. Of these, there are 180 protein families with multiple members, 312 families with exactly 37 members corresponding to core genes, 428 families with peripheral genes with varying taxonomic distribution and finally 1,125 smaller families. The fact that, even for smaller genomes of Chlamydiales, core genes represent over a quarter of the average protein complement, signifies a certain degree of structural stability, given the wide range of phylogenetic relationships within the group. In addition, the propagation of a corpus of manually curated annotations within the discovered core families reveals key functional properties, reflecting a coherent repertoire of cellular capabilities for Chlamydiales. We further investigate over 2,000 genes without homologs in the pangenome and discover two new protein sequence domains. Our results, supported by the genome-based phylogeny for this group, are fully consistent with previous analyses and current knowledge, and point to future research directions towards a better understanding of the structural and functional properties of Chlamydiales.

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