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da Silva EC, McManus CM, de Paiva Guimarães MP, et al. Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil. Genet Mol Biol. 2013;37(1):54-60doi: 10.1590/s1415-47572014000100010.
da Silva, E. C., McManus, C. M., de Paiva Guimarães, M. P., Gouveia, A. M., Facó, O., Pimentel, D. M., Caetano, A. R., & Paiva, S. R. (2014). Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil. Genetics and molecular biology, 37(1), 54-60. https://doi.org/10.1590/s1415-47572014000100010
da Silva, Elizabete Cristina, et al. "Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil." Genetics and molecular biology vol. 37,1 (2014): 54-60. doi: https://doi.org/10.1590/s1415-47572014000100010
da Silva EC, McManus CM, de Paiva Guimarães MP, Gouveia AM, Facó O, Pimentel DM, Caetano AR, Paiva SR. Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil. Genet Mol Biol. 2014 Mar;37(1):54-60. doi: 10.1590/s1415-47572014000100010. Epub 2013 Feb 28. PMID: 24688291; PMCID: PMC3958326.
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Genet Mol Biol. 2014 Mar;37(1):54-60. doi: 10.1590/s1415-47572014000100010. Epub 2013 Feb 28.

Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil.

Genetics and molecular biology

Elizabete Cristina da Silva, Concepta Margaret McManus, Maria Pia Souza Lima Mattos de Paiva Guimarães, Aurora M G Gouveia, Olivardo Facó, Daniel M Pimentel, Alexandre Rodrigues Caetano, Samuel Rezende Paiva

Affiliations

  1. Pós-graduação em Ciências Animais, Universidade de Brasília, Brasília, DF, Brazil . ; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil .
  2. Pós-graduação em Ciências Animais, Universidade de Brasília, Brasília, DF, Brazil . ; Departamento de Zootecnia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil .
  3. Caprileite/Associação dos Criadores de Caprinos e Ovinos de Minas Gerais, Belo Horizonte, MG, Brazil .
  4. Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil .
  5. Embrapa Caprinos e Ovinos, Sobral, CE, Brazil .
  6. Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Brasília, DF, Brazil .
  7. Pós-graduação em Ciências Animais, Universidade de Brasília, Brasília, DF, Brazil . ; Embrapa Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil . ; Embrapa Sede, Secretaria de Relações Internacionais, Brasília, DF, Brazil .

PMID: 24688291 PMCID: PMC3958326 DOI: 10.1590/s1415-47572014000100010

Abstract

Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided.

Keywords: Capra hircus; animal breeding; animal genetic resources; parentage errors

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