Display options
Cite
Su YC, Liu CT, Chu YL, et al. Eburicoic Acid, an Active Triterpenoid from the Fruiting Bodies of Basswood Cultivated Antrodia cinnamomea, Induces ER Stress-Mediated Autophagy in Human Hepatoma Cells. J Tradit Complement Med. 2012;2(4):312-22doi: 10.1016/s2225-4110(16)30117-1.
Su, Y. C., Liu, C. T., Chu, Y. L., Raghu, R., Kuo, Y. H., & Sheen, L. Y. (2012). Eburicoic Acid, an Active Triterpenoid from the Fruiting Bodies of Basswood Cultivated Antrodia cinnamomea, Induces ER Stress-Mediated Autophagy in Human Hepatoma Cells. Journal of traditional and complementary medicine, 2(4), 312-22. https://doi.org/10.1016/s2225-4110(16)30117-1
Su, Yu-Cheng, et al. "Eburicoic Acid, an Active Triterpenoid from the Fruiting Bodies of Basswood Cultivated Antrodia cinnamomea, Induces ER Stress-Mediated Autophagy in Human Hepatoma Cells." Journal of traditional and complementary medicine vol. 2,4 (2012): 312-22. doi: https://doi.org/10.1016/s2225-4110(16)30117-1
Su YC, Liu CT, Chu YL, Raghu R, Kuo YH, Sheen LY. Eburicoic Acid, an Active Triterpenoid from the Fruiting Bodies of Basswood Cultivated Antrodia cinnamomea, Induces ER Stress-Mediated Autophagy in Human Hepatoma Cells. J Tradit Complement Med. 2012 Oct;2(4):312-22. doi: 10.1016/s2225-4110(16)30117-1. PMID: 24716146; PMCID: PMC3942909.
Copy Download .nbib Format: NLM
Share it on

J Tradit Complement Med. 2012 Oct;2(4):312-22. doi: 10.1016/s2225-4110(16)30117-1.

Eburicoic Acid, an Active Triterpenoid from the Fruiting Bodies of Basswood Cultivated Antrodia cinnamomea, Induces ER Stress-Mediated Autophagy in Human Hepatoma Cells.

Journal of traditional and complementary medicine

Yu-Cheng Su, Chun-Ting Liu, Yung-Lin Chu, Rajasekaran Raghu, Yueh-Hsiung Kuo, Lee-Yan Sheen

Affiliations

  1. Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
  2. Tsuzuki Institute for Traditional Medicine, China Medical University, Taichung, Taiwan.

PMID: 24716146 PMCID: PMC3942909 DOI: 10.1016/s2225-4110(16)30117-1

Abstract

Antrodia cinnamomea, a Taiwan-specific medicinal mushroom, can manipulate biological activities, including hepatoprotection, anti-inflammation, anti-hepatitis B virus activity, anticancer activity, etc. In this study, the anti-liver cancer activity and molecular mechanisms of eburicoic acid, the second most abundant triterpenoid from the fruiting bodies of basswood cultivated Antrodia cinnamomea was investigated using the human hepatoma Hep 3B cells. The results show that eburicoic acid effectively reduced Hep 3B cell viability within 24 hours, and the IC50 was 18.4 μM, which was equivalent to 8.7 μg/mL. Besides, eburicoic acid induced conversion of LC3-I to LC3-II and a large number of autophagosomes/autolysosomes formation. In depth investigation for the molecular mechanisms, revealed that eburicoic acid firstly promoted reactive oxygen species generation and ATP depletion, leading to endoplasmic reticulum stress, followed by elevated cytosolic calcium ion concentration and BiP expression, downregulated phosphorylation of DAPK, upregulated phosphorylation of Beclin-1, JNK, and Bcl-2, and finally induced autophagy in Hep 3B cells. These results indicate that eburicoic acid has significant anti-liver cancer effects and more distinctive mechanisms.

Keywords: Antrodia cinnamomea; Autophagy; ER stress; Eburicoic acid; Liver cancer

References

  1. Food Chem Toxicol. 2006 Aug;44(8):1316-26 - PubMed
  2. Oncogene. 2007 Apr 2;26(15):2166-76 - PubMed
  3. Pediatr Nephrol. 2005 Jun;20(6):725-31 - PubMed
  4. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  5. Nature. 2008 Feb 28;451(7182):1069-75 - PubMed
  6. J Nat Prod. 2004 Dec;67(12):2008-11 - PubMed
  7. Nat Med. 2000 May;6(5):513-9 - PubMed
  8. Methods. 2008 Mar;44(3):205-21 - PubMed
  9. J Immunol Methods. 1993 Mar 15;160(1):81-8 - PubMed
  10. J Ethnopharmacol. 2009 Jan 21;121(2):194-212 - PubMed
  11. Antioxid Redox Signal. 2003 Aug;5(4):381-7 - PubMed
  12. Semin Liver Dis. 2005;25(2):155-61 - PubMed
  13. Cytometry. 1982 Nov;3(3):155-60 - PubMed
  14. Food Chem Toxicol. 2004 Dec;42(12):1937-47 - PubMed
  15. Autophagy. 2011 Jan;7(1):83-90 - PubMed
  16. FEBS J. 2011 Feb;278(3):403-13 - PubMed
  17. Circ Res. 2006 Aug 4;99(3):275-82 - PubMed
  18. J Immunol Methods. 1988 Nov 25;115(1):61-9 - PubMed
  19. J Immunol Methods. 1983 Dec 16;65(1-2):55-63 - PubMed
  20. Int J Cell Biol. 2010;2010:930509 - PubMed
  21. Br J Cancer. 1972 Aug;26(4):239-57 - PubMed
  22. Cancer Lett. 2009 Nov 18;285(1):73-9 - PubMed
  23. Planta Med. 2009 Dec;75(15):1549-60 - PubMed

Publication Types