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Wilder-Smith CH, Li X, Ho SS, et al. Fructose transporters GLUT5 and GLUT2 expression in adult patients with fructose intolerance. United European Gastroenterol J. 2014;2(1):14-21doi: 10.1177/2050640613505279.
Wilder-Smith, C. H., Li, X., Ho, S. S., Leong, S. M., Wong, R. K., Koay, E. S., & Ferraris, R. P. (2014). Fructose transporters GLUT5 and GLUT2 expression in adult patients with fructose intolerance. United European gastroenterology journal, 2(1), 14-21. https://doi.org/10.1177/2050640613505279
Wilder-Smith, Clive H, et al. "Fructose transporters GLUT5 and GLUT2 expression in adult patients with fructose intolerance." United European gastroenterology journal vol. 2,1 (2014): 14-21. doi: https://doi.org/10.1177/2050640613505279
Wilder-Smith CH, Li X, Ho SS, Leong SM, Wong RK, Koay ES, Ferraris RP. Fructose transporters GLUT5 and GLUT2 expression in adult patients with fructose intolerance. United European Gastroenterol J. 2014 Feb;2(1):14-21. doi: 10.1177/2050640613505279. PMID: 24918004; PMCID: PMC4040801.
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United European Gastroenterol J. 2014 Feb;2(1):14-21. doi: 10.1177/2050640613505279.

Fructose transporters GLUT5 and GLUT2 expression in adult patients with fructose intolerance.

United European gastroenterology journal

Clive H Wilder-Smith, Xinhua Li, Sherry Sy Ho, Sai Mun Leong, Reuben K Wong, Evelyn Sc Koay, Ronaldo P Ferraris

Affiliations

  1. Brain-Gut Research Group, Bern, Switzerland ; Neurogastroenterology Unit, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore.
  2. Neurogastroenterology Unit, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore.
  3. Molecular Diagnosis Centre, Department of Laboratory Medicine, National University Hospital, Singapore.
  4. Molecular Diagnosis Centre, Department of Laboratory Medicine, National University Hospital, Singapore ; Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore.
  5. Department of Pharmacology & Physiology, UMDNJ - New Jersey Medical School, Newark, USA.

PMID: 24918004 PMCID: PMC4040801 DOI: 10.1177/2050640613505279

Abstract

BACKGROUND: Gastrointestinal symptoms and malabsorption following fructose ingestion (fructose intolerance) are common in functional gastrointestinal disorders (FGID). The underlying mechanism is unclear, but is hypothesized to be related an abnormality of intestinal fructose transporter proteins.

OBJECTIVE: To assess the expression of the main intestinal fructose transporter proteins, glucose transport protein 5 (GLUT5) and 2 (GLUT2), in FGID.

METHODS: The expression of GLUT5 and GLUT2 protein and mRNA in small intestinal biopsy tissue was investigated using real-time reverse-transcription PCR and Western immunoblotting in 11 adults with FGID and fructose intolerance ascertained by breath testing and in 15 controls.

RESULTS: Median expression levels of GLUT5 mRNA normalized to beta-actin were 0.18 (interquartile range, IQR, 0.13-0.21) in patients and 0.17 (IQR 0.12-0.19) in controls (p > 0.05). Respective levels of GLUT2 mRNA were 0.26 (IQR 0.20-0.31) and 0.26 (IQR 0.19-0.31) (p > 0.05). Median expression levels of GLUT5 protein normalized to alpha-tubulin were 0.95 (IQR 0.52-1.68) in patients and 0.95 (IQR 0.59-1.15) in controls (p > 0.05). Respective protein expression levels for GLUT2 were 1.56 (IQR 1.06-2.14) and 1.35 (IQR 0.96-1.79) (p > 0.05).

CONCLUSIONS: Human fructose intolerance may not be associated with marked changes in GLUT5 and GLUT2 expression. Replication of these results in a larger subject group, including measures of transporter activation and membrane and subcellular localization, is warranted.

Keywords: FODMAP; GLUT2; GLUT5; fructose intolerance; fructose transporters; irritable bowel syndrome; malabsorption; visceral pain

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