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Lee SH, Chung MK, Sohn YJ, et al. Human Hair Follicle Cells with the Cell Surface Marker CD34 Can Regenerate New Mouse Hair Follicles and Located in the Outer Root Sheath of Immunodeficient Nude Mice. Int J Stem Cells. 2008;1(1):70-81doi: 10.15283/ijsc.2008.1.1.70.
Lee, S. H., Chung, M. K., Sohn, Y. J., Lee, Y. S., & Kang, K. S. (2008). Human Hair Follicle Cells with the Cell Surface Marker CD34 Can Regenerate New Mouse Hair Follicles and Located in the Outer Root Sheath of Immunodeficient Nude Mice. International journal of stem cells, 1(1), 70-81. https://doi.org/10.15283/ijsc.2008.1.1.70
Lee, Sung-Hoon, et al. "Human Hair Follicle Cells with the Cell Surface Marker CD34 Can Regenerate New Mouse Hair Follicles and Located in the Outer Root Sheath of Immunodeficient Nude Mice." International journal of stem cells vol. 1,1 (2008): 70-81. doi: https://doi.org/10.15283/ijsc.2008.1.1.70
Lee SH, Chung MK, Sohn YJ, Lee YS, Kang KS. Human Hair Follicle Cells with the Cell Surface Marker CD34 Can Regenerate New Mouse Hair Follicles and Located in the Outer Root Sheath of Immunodeficient Nude Mice. Int J Stem Cells. 2008 Nov;1(1):70-81. doi: 10.15283/ijsc.2008.1.1.70. PMID: 24855511; PMCID: PMC4021778.
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Int J Stem Cells. 2008 Nov;1(1):70-81. doi: 10.15283/ijsc.2008.1.1.70.

Human Hair Follicle Cells with the Cell Surface Marker CD34 Can Regenerate New Mouse Hair Follicles and Located in the Outer Root Sheath of Immunodeficient Nude Mice.

International journal of stem cells

Sung-Hoon Lee, Mi-Kyung Chung, Yong-Jin Sohn, Yong-Soon Lee, Kyung-Sun Kang

Affiliations

  1. Laboratory of Stem Cell and Tumor Biology, Department of Veterinary Public Health, College of Veterinary Medicine, and BK21 Program for Veterinary Science, Seoul National University ; Adult Stem Cell Research, Seoul, Korea.
  2. Modecell, Seoul, Korea.

PMID: 24855511 PMCID: PMC4021778 DOI: 10.15283/ijsc.2008.1.1.70

Abstract

BACKGROUND AND OBJECTIVES: The bulge region of hair follicle has been reported as a putative reservoir of hair follicle stem cells. The purpose of this study was to compare hair follice CD34 negative (CD34-) cell with CD34 positive (CD34+) cell and to evaluate the ability to regenerate new hair of immunodeficient nude mouse.

METHODS AND RESULTS: In this report, we isolated the cells with CD34, known as bulge-negative cell surface marker from cultured human hair follicle cells using by magnetic cell sorting (MACS), injected the cells to immunodeficient nude mouse. To determine immunological characterization, human hair follicle CD34+ cells and CD34- cells were assessed by flow cytometry. The localization of injected-CD34+ cells was assessed on formalin-fixed, paraffin-embedded mouse skin samples by in situ hybridization technique. Our findings show that the human hair follicle cells with cell surface marker CD34 were located in the outer root sheath of nude mouse after transplantation and the cells were able to regenerate new hair follicle in immunodeficient nude mouse. CD34- cells also were able to regenerate follicles in the mouse, however, CD34+ cells were able to regenerate much more hair follicle than CD34- cells.

CONCLUSIONS: Therefore, the results of this study add new insight into the investigation of CD34 stem cell-related molecule in human hair follicles and suggest that not all human hair follicle stem cells reside in bulge region, but in a lager niche.

Keywords: Bulge; CD34; Hair follicle; Hair follicle stem cells; Stem cell niche

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