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Chatterjee S, Chaklader M, Basak P, et al. An animal model of chronic aplastic bone marrow failure following pesticide exposure in mice. Int J Stem Cells. 2010;3(1):54-62doi: 10.15283/ijsc.2010.3.1.54.
Chatterjee, S., Chaklader, M., Basak, P., Das, P., Das, M., Pereira, J. A., Dutta, R. K., Chaudhuri, S., & Law, S. (2010). An animal model of chronic aplastic bone marrow failure following pesticide exposure in mice. International journal of stem cells, 3(1), 54-62. https://doi.org/10.15283/ijsc.2010.3.1.54
Chatterjee, Sumanta, et al. "An animal model of chronic aplastic bone marrow failure following pesticide exposure in mice." International journal of stem cells vol. 3,1 (2010): 54-62. doi: https://doi.org/10.15283/ijsc.2010.3.1.54
Chatterjee S, Chaklader M, Basak P, Das P, Das M, Pereira JA, Dutta RK, Chaudhuri S, Law S. An animal model of chronic aplastic bone marrow failure following pesticide exposure in mice. Int J Stem Cells. 2010 May;3(1):54-62. doi: 10.15283/ijsc.2010.3.1.54. PMID: 24855541; PMCID: PMC4022690.
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Int J Stem Cells. 2010 May;3(1):54-62. doi: 10.15283/ijsc.2010.3.1.54.

An animal model of chronic aplastic bone marrow failure following pesticide exposure in mice.

International journal of stem cells

Sumanta Chatterjee, Malay Chaklader, Pratima Basak, Prosun Das, Madhurima Das, Jacintha Archana Pereira, Ranjan Kumar Dutta, Samaresh Chaudhuri, Sujata Law

Affiliations

  1. Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, 108, C.R Avenue, Kolkata-700073, India.

PMID: 24855541 PMCID: PMC4022690 DOI: 10.15283/ijsc.2010.3.1.54

Abstract

The wide use of pesticides for agriculture, domestic and industrial purposes and evaluation of their subsequent effect is of major concern for public health. Human exposure to these contaminants especially bone marrow with its rapidly renewing cell population is one of the most sensitive tissues to these toxic agents represents a risk for the immune system leading to the onset of different pathologies. In this experimental protocol we have developed a mouse model of pesticide(s) induced hypoplastic/aplastic marrow failure to study quantitative changes in the bone marrow hematopoietic stem cell (BMHSC) population through flowcytometric analysis, defects in the stromal microenvironment through short term adherent cell colony (STACC) forming assay and immune functional capacity of the bone marrow derived cells through cell mediated immune (CMI) parameter study. A time course dependent analysis for consecutive 90 days were performed to monitor the associated changes in the marrow's physiology after 30(th), 60(th) and 90(th) days of chronic pesticide exposure. The peripheral blood showed maximum lowering of the blood cell count after 90 days which actually reflected the bone marrow scenario. Severe depression of BMHSC population, immune profile of the bone marrow derived cells and reduction of adherent cell colonies pointed towards an essentially empty and hypoplastic marrow condition that resembled the disease aplastic anemia. The changes were accompanied by splenomegaly and splenic erythroid hyperplasia. In conclusion, this animal model allowed us a better understanding of clinico-biological findings of the disease aplastic anemia following toxic exposure to the pesticide(s) used for agricultural and industrial purposes.

Keywords: Adherent stromal cells; Aplastic anemia; Bone marrow failure; Pesticide; Stem cells

References

  1. J Occup Med. 1981 May;23(5):364-6 - PubMed
  2. JAMA. 1965 Jul 12;193:110-4 - PubMed
  3. Toxicol Ind Health. 1992 Sep-Oct;8(5):239-54 - PubMed
  4. Ann Intern Med. 2002 Apr 2;136(7):534-46 - PubMed
  5. Exp Hematol. 1980 Jul;8(6):788-94 - PubMed
  6. J Clin Pathol. 1990 Feb;43(2):98-101 - PubMed
  7. N Engl J Med. 1963 Dec 19;269:1365-7 - PubMed
  8. Int J Radiat Oncol Biol Phys. 1979 Sep;5(9):1621-5 - PubMed
  9. Arch Toxicol. 1991;65(7):556-61 - PubMed
  10. J Stem Cells. 2009;4(3):179-89 - PubMed
  11. Blood. 1976 Jul;48(1):71-6 - PubMed
  12. Cancer Res. 1977 Apr;37(4):1205-9 - PubMed
  13. Health Policy Plan. 1997 Jun;12(2):97-106 - PubMed
  14. Toxicol Lett. 1991 Apr;56(1-2):159-66 - PubMed
  15. Int J Hematol. 2005 Oct;82(3):196-200 - PubMed
  16. J Occup Med. 1993 Nov;35(11):1106-16 - PubMed
  17. Blood. 1973 Feb;41(2):187-95 - PubMed
  18. Blood. 1975 May;45(5):681-8 - PubMed
  19. Clin Med Res. 2005 May;3(2):102-8 - PubMed
  20. Toxicol Appl Pharmacol. 1995 Sep;134(1):53-62 - PubMed
  21. Blood. 1984 Nov;64(5):1036-41 - PubMed
  22. Exp Hematol. 1978 Nov;6(10):791-800 - PubMed
  23. Drug Chem Toxicol. 2004 May;27(2):133-44 - PubMed
  24. Exp Hematol. 1994 Jul;22(7):573-81 - PubMed
  25. Stem Cells Int. 2010;2010:614395 - PubMed

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