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Suhovskih AV, Tsidulko AY, Kutsenko OS, et al. Transcriptional Activity of Heparan Sulfate Biosynthetic Machinery is Specifically Impaired in Benign Prostate Hyperplasia and Prostate Cancer. Front Oncol. 2014;4:79doi: 10.3389/fonc.2014.00079.
Suhovskih, A. V., Tsidulko, A. Y., Kutsenko, O. S., Kovner, A. V., Aidagulova, S. V., Ernberg, I., & Grigorieva, E. V. (2014). Transcriptional Activity of Heparan Sulfate Biosynthetic Machinery is Specifically Impaired in Benign Prostate Hyperplasia and Prostate Cancer. Frontiers in oncology, 479. https://doi.org/10.3389/fonc.2014.00079
Suhovskih, Anastasia V, et al. "Transcriptional Activity of Heparan Sulfate Biosynthetic Machinery is Specifically Impaired in Benign Prostate Hyperplasia and Prostate Cancer." Frontiers in oncology vol. 4 (2014): 79. doi: https://doi.org/10.3389/fonc.2014.00079
Suhovskih AV, Tsidulko AY, Kutsenko OS, Kovner AV, Aidagulova SV, Ernberg I, Grigorieva EV. Transcriptional Activity of Heparan Sulfate Biosynthetic Machinery is Specifically Impaired in Benign Prostate Hyperplasia and Prostate Cancer. Front Oncol. 2014 Apr 15;4:79. doi: 10.3389/fonc.2014.00079. eCollection 2014. PMID: 24782989; PMCID: PMC3995048.
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Front Oncol. 2014 Apr 15;4:79. doi: 10.3389/fonc.2014.00079. eCollection 2014.

Transcriptional Activity of Heparan Sulfate Biosynthetic Machinery is Specifically Impaired in Benign Prostate Hyperplasia and Prostate Cancer.

Frontiers in oncology

Anastasia V Suhovskih, Alexandra Y Tsidulko, Olesya S Kutsenko, Anna V Kovner, Svetlana V Aidagulova, Ingemar Ernberg, Elvira V Grigorieva

Affiliations

  1. Institute of Molecular Biology and Biophysics SD RAMS , Novosibirsk , Russia ; Novosibirsk State University , Novosibirsk , Russia.
  2. Institute of Molecular Biology and Biophysics SD RAMS , Novosibirsk , Russia.
  3. Research Center of Clinical and Experimental Medicine SD RAMS , Novosibirsk , Russia.
  4. Novosibirsk State Medical University , Novosibirsk , Russia.
  5. MTC, Karolinska Institute , Stockholm , Sweden.
  6. Institute of Molecular Biology and Biophysics SD RAMS , Novosibirsk , Russia ; MTC, Karolinska Institute , Stockholm , Sweden.

PMID: 24782989 PMCID: PMC3995048 DOI: 10.3389/fonc.2014.00079

Abstract

Heparan sulfates (HSs) are key components of mammalian cells surface and extracellular matrix. Structure and composition of HS, generated by HS-biosynthetic system through non-template-driven process, are significantly altered in cancer tissues. The aim of this study was to investigate the involvement of HS-metabolic machinery in prostate carcinogenesis. Transcriptional patterns of HS-metabolic enzymes (EXT1, EXT2, NDST1, NDST2, GLCE, 3OST1/HS3ST1, SULF1, SULF2, HPSE) were determined in normal, benign, and cancer human prostate tissues and cell lines (PNT2, LNCaP, PC3, DU145). Stability of the HS-metabolic system patterns under the pressure of external or internal stimuli was studied. Overall impairment of transcriptional activity of HS-metabolic machinery was detected in benign prostate hyperplasia, while both significant decrease in the transcriptional activity and changes in the expression patterns of HS metabolism-involved genes were observed in prostate tumors. Prostate cancer cell lines possessed specific transcriptional patterns of HS metabolism-involved genes; however, expression activity of the system was similar to that of normal prostate PNT2 cells. HS-metabolic system was able to dynamically react to different external or internal stimuli in a cell type-dependent manner. LNCaP cells were sensitive to the external stimuli (5-aza-deoxycytidin or Trichostatin A treatments; co-cultivation with human fibroblasts), whereas PC3 cells almost did not respond to the treatments. Ectopic GLCE over-expression resulted in transcriptional activation of HS-biosynthetic machinery in both cell lines, suggesting an existence of a self-regulating mechanism for the coordinated transcription of HS metabolism-involved genes. Taken together, these findings demonstrate impairment of HS-metabolic system in prostate tumors in vivo but not in prostate cancer cells in vitro, and suggest that as a potential microenvironmental biomarker for prostate cancer diagnostics and treatment.

Keywords: biosynthesis; expression; heparan sulfate; prostate cancer; proteoglycan; transcriptional pattern

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