Display options
Cite
Huang Y, Zhao B, Liu Y, et al. Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells. PPAR Res. 2014;2014:740121doi: 10.1155/2014/740121.
Huang, Y., Zhao, B., Liu, Y., & Wang, N. (2014). Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells. PPAR research, 2014740121. https://doi.org/10.1155/2014/740121
Huang, Yazi, et al. "Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells." PPAR research vol. 2014 (2014): 740121. doi: https://doi.org/10.1155/2014/740121
Huang Y, Zhao B, Liu Y, Wang N. Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells. PPAR Res. 2014;2014:740121. doi: 10.1155/2014/740121. Epub 2014 May 12. PMID: 24955089; PMCID: PMC4052932.
Copy Download .nbib Format: NLM
Share it on

PPAR Res. 2014;2014:740121. doi: 10.1155/2014/740121. Epub 2014 May 12.

Peroxisome Proliferator-Activated Receptor γ Regulates the Expression of Lipid Phosphate Phosphohydrolase 1 in Human Vascular Endothelial Cells.

PPAR research

Yazi Huang, Beilei Zhao, Yahan Liu, Nanping Wang

Affiliations

  1. Institute of Cardiovascular Science, Peking University Health Science Center, Beijing 100191, China.

PMID: 24955089 PMCID: PMC4052932 DOI: 10.1155/2014/740121

Abstract

Lipid phosphate phosphohydrolase 1 (LPP1), a membrane ectophosphohydrolase regulating the availability of bioactive lipid phosphates, plays important roles in cellular signaling and physiological processes such as angiogenesis and endothelial migration. However, the regulated expression of LPP1 remains largely unknown. Here, we aimed to examine a role of peroxisome proliferator-activated receptor γ (PPAR γ ) in the transcriptional control of LPP1 gene expression. In human umbilical vein endothelial cells (HUVECs), quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) demonstrated that activation of PPAR γ increased the mRNA level of LPP1. Chromatin immunoprecipitation assay showed that PPAR γ binds to the putative PPAR-responsive elements (PPREs) within the 5'-flanking region of the human LPP1 gene. Genomic fragment containing 1.7-kilobase of the promoter region was cloned by using PCR. The luciferase reporter assays demonstrated that overexpression of PPAR γ and rosiglitazone, a specific ligand for PPAR γ , could significantly upregulate the reporter activity. However, site-directed mutagenesis of the PPRE motif abolished the induction. In conclusion, our results demonstrated that PPAR γ transcriptionally activated the expression of LPP1 gene in ECs, suggesting a potential role of PPAR γ in the metabolism of phospholipids.

References

  1. Trends Cardiovasc Med. 2002 Nov;12(8):354-62 - PubMed
  2. Cardiovasc Hematol Disord Drug Targets. 2007 Jun;7(2):109-17 - PubMed
  3. Arterioscler Thromb Vasc Biol. 2009 Jan;29(1):67-74 - PubMed
  4. J Biol Chem. 1995 Jun 2;270(22):12953-6 - PubMed
  5. Biochim Biophys Acta. 2002 May 23;1582(1-3):45-51 - PubMed
  6. Endocrinology. 1996 Jan;137(1):354-66 - PubMed
  7. Arterioscler Thromb Vasc Biol. 2001 Sep;21(9):1414-20 - PubMed
  8. J Biol Chem. 2002 Sep 13;277(37):34176-81 - PubMed
  9. Int J Obes (Lond). 2005 Mar;29 Suppl 1:S13-6 - PubMed
  10. Circulation. 2003 Aug 12;108(6):741-7 - PubMed
  11. J Biol Chem. 1998 Sep 18;273(38):24281-4 - PubMed
  12. Biochim Biophys Acta. 1992 Apr 8;1125(1):110-2 - PubMed
  13. J Biol Chem. 1996 Jul 12;271(28):16506-9 - PubMed
  14. Ann N Y Acad Sci. 2006 May;1067:448-53 - PubMed
  15. J Biol Chem. 1998 Feb 20;273(8):4660-5 - PubMed
  16. Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):131-6 - PubMed
  17. Ann N Y Acad Sci. 1996 Dec 27;804:231-51 - PubMed
  18. Circulation. 2000 Jan 25;101(3):235-8 - PubMed
  19. Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6931-6 - PubMed
  20. J Biol Chem. 1998 Aug 21;273(34):22059-67 - PubMed
  21. Vitam Horm. 2003;66:157-88 - PubMed
  22. Vascul Pharmacol. 2006 Jul;45(1):19-28 - PubMed
  23. Cell. 1995 Dec 1;83(5):803-12 - PubMed
  24. EMBO J. 1996 Oct 1;15(19):5336-48 - PubMed
  25. Curr Med Chem. 2006;13(26):3227-38 - PubMed
  26. Cell Metab. 2011 May 4;13(5):592-600 - PubMed
  27. Biochim Biophys Acta. 2002 May 23;1582(1-3):216-21 - PubMed
  28. Arterioscler Thromb Vasc Biol. 2008 Feb;28(2):315-21 - PubMed
  29. Pharmacol Rev. 2002 Jun;54(2):265-9 - PubMed
  30. Clin Cancer Res. 2003 Jul;9(7):2866-75 - PubMed
  31. Biochem J. 1997 Jun 15;324 ( Pt 3):941-50 - PubMed

Publication Types