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Sánchez MI, Penas C, Vázquez ME, et al. Metal-catalyzed uncaging of DNA-binding agents in living cells. Chem Sci. 2014;2014(5):1901-1907
Sánchez, M. I., Penas, C., Vázquez, M. E., & Mascareñas, J. L. (2014). Metal-catalyzed uncaging of DNA-binding agents in living cells. Chemical science, 2014(5), 1901-1907.
Sánchez, Mateo I, et al. "Metal-catalyzed uncaging of DNA-binding agents in living cells." Chemical science vol. 2014,5 (2014): 1901-1907.
Sánchez MI, Penas C, Vázquez ME, Mascareñas JL. Metal-catalyzed uncaging of DNA-binding agents in living cells. Chem Sci. 2014 May 01;2014(5):1901-1907. PMID: 24955233; PMCID: PMC4064253.
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Chem Sci. 2014 May 01;2014(5):1901-1907.

Metal-catalyzed uncaging of DNA-binding agents in living cells.

Chemical science

Mateo I Sánchez, Cristina Penas, M Eugenio Vázquez, José L Mascareñas

Affiliations

  1. Departamento de Química Orgánica e Centro Singular de Investigación en Química Biolóxica y Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.

PMID: 24955233 PMCID: PMC4064253

Abstract

Attachment of alloc protecting groups to the amidine units of fluorogenic DNA-binding bisbenzamidines or to the amino groups of ethidium bromide leads to a significant reduction of their DNA affinity. More importantly, the active DNA-binding species can be readily regenerated by treatment with ruthenium catalysts in aqueous conditions, even in cell cultures. The catalytic chemical uncaging can be easily monitored by fluorescence microscopy, because the protected products display both different emission properties and cell distribution to the parent compounds.

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