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Kakuta S, Bando Y, Nishiumi S, et al. Metabolic profiling of oxidized lipid-derived volatiles in blood by gas chromatography/mass spectrometry with in-tube extraction. Mass Spectrom (Tokyo). 2013;2(1):A0018doi: 10.5702/massspectrometry.A0018.
Kakuta, S., Bando, Y., Nishiumi, S., Yoshida, M., Fukusaki, E., & Bamba, T. (2013). Metabolic profiling of oxidized lipid-derived volatiles in blood by gas chromatography/mass spectrometry with in-tube extraction. Mass spectrometry (Tokyo, Japan), 2(1), A0018. https://doi.org/10.5702/massspectrometry.A0018
Kakuta, Shoji, et al. "Metabolic profiling of oxidized lipid-derived volatiles in blood by gas chromatography/mass spectrometry with in-tube extraction." Mass spectrometry (Tokyo, Japan) vol. 2,1 (2013): A0018. doi: https://doi.org/10.5702/massspectrometry.A0018
Kakuta S, Bando Y, Nishiumi S, Yoshida M, Fukusaki E, Bamba T. Metabolic profiling of oxidized lipid-derived volatiles in blood by gas chromatography/mass spectrometry with in-tube extraction. Mass Spectrom (Tokyo). 2013;2(1):A0018. doi: 10.5702/massspectrometry.A0018. Epub 2013 Apr 26. PMID: 24860708; PMCID: PMC3967009.
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Mass Spectrom (Tokyo). 2013;2(1):A0018. doi: 10.5702/massspectrometry.A0018. Epub 2013 Apr 26.

Metabolic profiling of oxidized lipid-derived volatiles in blood by gas chromatography/mass spectrometry with in-tube extraction.

Mass spectrometry (Tokyo, Japan)

Shoji Kakuta, Yasuhiko Bando, Shin Nishiumi, Masaru Yoshida, Eiichiro Fukusaki, Takeshi Bamba

Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Osaka University.
  2. AMR Inc.
  3. Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine.
  4. Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine ; The Integrated Center for Mass Spectrometry, Kobe University Graduate School of Medicine ; Division of Metabolomics Research, Kobe University Graduate School of Medicine.

PMID: 24860708 PMCID: PMC3967009 DOI: 10.5702/massspectrometry.A0018

Abstract

Once lipids are oxidized, various volatiles are produced by cleavage of the fatty acid side chain. Considering the variety of lipids present in the body, a large number of possible volatiles might originate from oxidized lipids. However, only specific volatiles such as aldehydes are exclusively examined in current studies, and there is no reported method for the exhaustive analysis of all volatiles. We developed a sensitive analytical method for the detection of all possible volatiles for multimarker profiling, applying a new extraction method called in-tube extraction. Oxidized phosphatidyl choline standards were prepared in vitro and analyzed in order to determine the potential variety of volatiles. Over 40 compounds, including alcohols, ketones, and furanones, were identified in addition to the aldehydes reported previously. Based on this result, we applied our analytical method to mouse plasma and identified 12 volatiles, including 1-octen-3-ol, which is correlated to disease states. To determine the volatile profile after oxidation, we oxidized plasma in vitro under various conditions and identified 27 volatiles, including 1-octen-3-ol and benzaldehyde. The generation capacity of each volatile was different. This method allows sensitive and exhaustive analysis of various volatiles in addition to aldehydes.

Keywords: in tube extraction; lipids; metabolic profiling; oxidation; volatile

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