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Carlier-Grynkorn F, Ji L, Fraisier V, et al. Fission yeast mtr1p regulates interphase microtubule cortical dwell-time. Biol Open. 2014;3(7):591-6doi: 10.1242/bio.20148607.
Carlier-Grynkorn, F., Ji, L., Fraisier, V., Lombard, B., Dingli, F., Loew, D., Paoletti, A., Ronot, X., & Tran, P. T. (2014). Fission yeast mtr1p regulates interphase microtubule cortical dwell-time. Biology open, 3(7), 591-6. https://doi.org/10.1242/bio.20148607
Carlier-Grynkorn, Frédérique, et al. "Fission yeast mtr1p regulates interphase microtubule cortical dwell-time." Biology open vol. 3,7 (2014): 591-6. doi: https://doi.org/10.1242/bio.20148607
Carlier-Grynkorn F, Ji L, Fraisier V, Lombard B, Dingli F, Loew D, Paoletti A, Ronot X, Tran PT. Fission yeast mtr1p regulates interphase microtubule cortical dwell-time. Biol Open. 2014 Jun 13;3(7):591-6. doi: 10.1242/bio.20148607. PMID: 24928430; PMCID: PMC4154295.
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Biol Open. 2014 Jun 13;3(7):591-6. doi: 10.1242/bio.20148607.

Fission yeast mtr1p regulates interphase microtubule cortical dwell-time.

Biology open

Frédérique Carlier-Grynkorn, Liang Ji, Vincent Fraisier, Berangère Lombard, Florent Dingli, Damarys Loew, Anne Paoletti, Xavier Ronot, Phong T Tran

Affiliations

  1. Institut Curie, Paris 75005, France CNRS, UMR 144, Paris 75005, France.
  2. Institut Curie, Paris 75005, France.
  3. Laboratoire CaCyS, FRE AGIM 3405 UJF-CNRS-EPHE-UMPF, La Tronche 38700, France.
  4. Institut Curie, Paris 75005, France CNRS, UMR 144, Paris 75005, France Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA [email protected].

PMID: 24928430 PMCID: PMC4154295 DOI: 10.1242/bio.20148607

Abstract

The microtubule cytoskeleton plays important roles in cell polarity, motility and division. Microtubules inherently undergo dynamic instability, stochastically switching between phases of growth and shrinkage. In cells, some microtubule-associated proteins (MAPs) and molecular motors can further modulate microtubule dynamics. We present here the fission yeast mtr1(+), a new regulator of microtubule dynamics that appears to be not a MAP or a motor. mtr1-deletion (mtr1Δ) primarily results in longer microtubule dwell-time at the cell tip cortex, suggesting that mtr1p acts directly or indirectly as a destabilizer of microtubules. mtr1p is antagonistic to mal3p, the ortholog of mammalian EB1, which stabilizes microtubules. mal3Δ results in short microtubules, but can be partially rescued by mtr1Δ, as the double mutant mal3Δ mtr1Δ exhibits longer microtubules than mal3Δ single mutant. By sequence homology, mtr1p is predicted to be a component of the ribosomal quality control complex. Intriguingly, deletion of a predicted ribosomal gene, rps1801, also resulted in longer microtubule dwell-time similar to mtr1Δ. The double-mutant mal3Δ rps1801Δ also exhibits longer microtubules than mal3Δ single mutant alone. Our study suggests a possible involvement of mtr1p and the ribosome complex in modulating microtubule dynamics.

© 2014. Published by The Company of Biologists Ltd.

Keywords: Dynamic instability; Microtubule; Ribosome

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