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Wu F, Song Y, Li F, et al. Wen-dan decoction improves negative emotions in sleep-deprived rats by regulating orexin-a and leptin expression. Evid Based Complement Alternat Med. 2014;2014:872547doi: 10.1155/2014/872547.
Wu, F., Song, Y., Li, F., He, X., Ma, J., Feng, T., Guan, B., Wang, L., Li, S., Liu, X., Liu, Y., Mao, M., Liu, J., Bai, S., & Song, C. (2014). Wen-dan decoction improves negative emotions in sleep-deprived rats by regulating orexin-a and leptin expression. Evidence-based complementary and alternative medicine : eCAM, 2014872547. https://doi.org/10.1155/2014/872547
Wu, Fengzhi, et al. "Wen-dan decoction improves negative emotions in sleep-deprived rats by regulating orexin-a and leptin expression." Evidence-based complementary and alternative medicine : eCAM vol. 2014 (2014): 872547. doi: https://doi.org/10.1155/2014/872547
Wu F, Song Y, Li F, He X, Ma J, Feng T, Guan B, Wang L, Li S, Liu X, Liu Y, Mao M, Liu J, Bai S, Song C. Wen-dan decoction improves negative emotions in sleep-deprived rats by regulating orexin-a and leptin expression. Evid Based Complement Alternat Med. 2014;2014:872547. doi: 10.1155/2014/872547. Epub 2014 Apr 17. PMID: 24864160; PMCID: PMC4016855.
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Evid Based Complement Alternat Med. 2014;2014:872547. doi: 10.1155/2014/872547. Epub 2014 Apr 17.

Wen-dan decoction improves negative emotions in sleep-deprived rats by regulating orexin-a and leptin expression.

Evidence-based complementary and alternative medicine : eCAM

Fengzhi Wu, Yuehan Song, Feng Li, Xin He, Jie Ma, Ting Feng, Binghe Guan, Liye Wang, Sinai Li, Xiaolan Liu, Yan Liu, Meng Mao, Jing Liu, Shijing Bai, Cai Song

Affiliations

  1. School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 Bei Sanhuan Donglu, Chaoyang District, Beijing 100029, China.
  2. Traditional Chinese Medical Hospital of Ji'nan, Shandong 250012, China.
  3. School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong.
  4. Graduate Institute of Neural and Cognitive Sciences, China Medical University and CMU Hospital, Taichung 40402, Taiwan ; Research Institute for Marine Drugs and Nutrition, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.

PMID: 24864160 PMCID: PMC4016855 DOI: 10.1155/2014/872547

Abstract

Wen-Dan Decoction (WDD), a formula of traditional Chinese medicine, has been clinically used for treating insomnia for approximately 800 years. However, the therapeutic mechanisms of WDD remain unclear. Orexin-A plays a key role in the sleep-wake cycle, while leptin function is opposite to orexin-A. Thus, orexin-A and leptin may be important factors in sleep disorders. In this study, 48 rats were divided into control, model, WDD-treated, and diazepam-treated groups. The model of insomnia was produced by sleep deprivation (SD) for 14 days. The expressions of orexin-A, leptin, and their receptors in blood serum, prefrontal cortex, and hypothalamus were detected by enzyme-linked immunosorbent assay, immunohistochemistry, and real time PCR. Open field tests showed that SD increased both crossing movement (Cm) and rearing-movement (Rm) times. Orexin-A and leptin levels in blood serum increased after SD but decreased in brain compared to the control group. mRNA expressions of orexin receptor 1 and leptin receptor after SD were decreased in the prefrontal cortex but were increased in hypothalamus. WDD treatment normalized the behavior and upregulated orexin-A, leptin, orexin receptor 1 and leptin receptor in brain. The findings suggest that WDD treatment may regulate SD-induced negative emotions by regulating orexin-A and leptin expression.

References

  1. Behav Brain Res. 2009 Feb 11;197(2):450-3 - PubMed
  2. Anesth Analg. 2009 Feb;108(2):491-5 - PubMed
  3. J Endocrinol. 2006 Oct;191(1):129-36 - PubMed
  4. JAMA. 2012 Jun 27;307(24):2653 - PubMed
  5. Psychiatry Investig. 2013 Dec;10(4):373-81 - PubMed
  6. Physiol Behav. 2000 Jan;68(3):309-16 - PubMed
  7. Neurochem Res. 2013 Apr;38(4):726-31 - PubMed
  8. J Anesth. 2011 Feb;25(1):65-71 - PubMed
  9. J Comp Neurol. 2001 Jun 18;435(1):6-25 - PubMed
  10. Neurosci Biobehav Rev. 2001 May;25(3):235-60 - PubMed
  11. Cell. 1998 Feb 20;92(4):573-85 - PubMed
  12. J Clin Sleep Med. 2007 Aug 15;3(5 Suppl):S7-10 - PubMed
  13. Disabil Rehabil. 2014;36(20):1661-9 - PubMed
  14. Proc Natl Acad Sci U S A. 2011 Mar 15;108(11):4471-6 - PubMed
  15. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1726-30 - PubMed
  16. J Neurosci. 2004 Jul 14;24(28):6291-300 - PubMed
  17. Ann Intern Med. 2004 Dec 7;141(11):846-50 - PubMed
  18. J Neurosci. 2009 Nov 18;29(46):14423-38 - PubMed
  19. Behav Brain Res. 2012 Mar 17;228(2):311-8 - PubMed
  20. Sleep Med Rev. 2010 Aug;14(4):227-38 - PubMed
  21. Pharmazie. 2012 Mar;67(3):260-3 - PubMed
  22. Eur J Pharmacol. 2003 Feb 28;463(1-3):3-33 - PubMed
  23. Neuroscience. 2001;103(3):777-97 - PubMed
  24. Eur J Neurosci. 2001 Oct;14(7):1075-81 - PubMed

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