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Pranaw K, Singh S, Dutta D, et al. Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3. Biotechnol Res Int. 2014;2014:293434doi: 10.1155/2014/293434.
Pranaw, K., Singh, S., Dutta, D., Chaudhuri, S., Ganguly, S., & Nain, L. (2014). Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3. Biotechnology research international, 2014293434. https://doi.org/10.1155/2014/293434
Pranaw, Kumar, et al. "Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3." Biotechnology research international vol. 2014 (2014): 293434. doi: https://doi.org/10.1155/2014/293434
Pranaw K, Singh S, Dutta D, Chaudhuri S, Ganguly S, Nain L. Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3. Biotechnol Res Int. 2014;2014:293434. doi: 10.1155/2014/293434. Epub 2014 Apr 23. PMID: 24864214; PMCID: PMC4017731.
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Biotechnol Res Int. 2014;2014:293434. doi: 10.1155/2014/293434. Epub 2014 Apr 23.

Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3.

Biotechnology research international

Kumar Pranaw, Surender Singh, Debjani Dutta, Surabhi Chaudhuri, Sudershan Ganguly, Lata Nain

Affiliations

  1. Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India ; Department of Biotechnology, National Institute of Technology, Durgapur 713209, India.
  2. Division of Microbiology, Indian Agricultural Research Institute, New Delhi 110012, India.
  3. Department of Biotechnology, National Institute of Technology, Durgapur 713209, India.
  4. Division of Nematology, Indian Agricultural Research Institute, New Delhi 110012, India.

PMID: 24864214 PMCID: PMC4017731 DOI: 10.1155/2014/293434

Abstract

Xenorhabdus indica KB-3, a well-known protease producer, was isolated from its entomopathogenic nematode symbiont Steinernema thermophilum. Since medium constituents are critical to the protease production, the chemical components of the selected medium (soya casein digest broth) were optimized by rotatable central composite design (RCCD) using response surface methodology (RSM). The effects of all five chemical components (considered as independent variables), namely tryptone, soya peptone, dextrose, NaCl, and dipotassium phosphate, on protease production (dependent variable) were studied, and it was found that tryptone and dextrose had maximum influence on protease production. The protease production was increased significantly by 66.31% under optimal medium conditions (tryptone-5.71, soya peptone-4.9, dextrose-1.45, NaCl-6.08, and dipotassium phosphate-0.47 in g/L). To best of knowledge, there are no reports on optimization of medium component for protease production by X. indica KB-3 using RSM and their application in fibrinolysis. This study will be useful for industrial processes for production of protease enzyme from X. indica KB-3 for its application in the field of agriculture and medicine.

References

  1. J Microbiol Biotechnol. 2013 Nov 28;23(11):1536-43 - PubMed
  2. Curr Opin Microbiol. 2012 Jun;15(3):220-31 - PubMed
  3. Talanta. 2008 Sep 15;76(5):965-77 - PubMed
  4. Int J Biol Macromol. 2013 Apr;55:169-75 - PubMed
  5. Appl Environ Microbiol. 2002 Mar;68(3):1297-304 - PubMed
  6. Biochim Biophys Acta. 2011 Oct;1814(10):1333-9 - PubMed
  7. J Biosci Bioeng. 2012 Mar;113(3):307-14 - PubMed
  8. J Microbiol Biotechnol. 2010 May;20(5):950-7 - PubMed
  9. J Pharmacol Pharmacother. 2010 Jul;1(2):116-7 - PubMed
  10. Int J Biochem Cell Biol. 1999 Dec;31(12):1435-41 - PubMed
  11. FEBS J. 2007 Apr;274(8):1946-56 - PubMed
  12. PLoS One. 2012;7(6):e38421 - PubMed
  13. J Chromatogr A. 2007 Jul 27;1158(1-2):2-14 - PubMed
  14. Appl Environ Microbiol. 2009 May;75(10):3120-6 - PubMed
  15. J Gen Microbiol. 1988 Jul;134(7):1835-45 - PubMed
  16. Arch Biochem Biophys. 1995 Mar 10;317(2):365-73 - PubMed
  17. Bioresour Technol. 2010 Mar;101(6):1954-60 - PubMed

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