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van Steenwyk G, Janeczek P, Lewohl JM. Differential Effects of Chronic and Chronic-Intermittent Ethanol Treatment and Its Withdrawal on the Expression of miRNAs. Brain Sci. 2013;3(2):744-56doi: 10.3390/brainsci3020744.
van Steenwyk, G., Janeczek, P., & Lewohl, J. M. (2013). Differential Effects of Chronic and Chronic-Intermittent Ethanol Treatment and Its Withdrawal on the Expression of miRNAs. Brain sciences, 3(2), 744-56. https://doi.org/10.3390/brainsci3020744
van Steenwyk, Gretchen, et al. "Differential Effects of Chronic and Chronic-Intermittent Ethanol Treatment and Its Withdrawal on the Expression of miRNAs." Brain sciences vol. 3,2 (2013): 744-56. doi: https://doi.org/10.3390/brainsci3020744
van Steenwyk G, Janeczek P, Lewohl JM. Differential Effects of Chronic and Chronic-Intermittent Ethanol Treatment and Its Withdrawal on the Expression of miRNAs. Brain Sci. 2013 May 03;3(2):744-56. doi: 10.3390/brainsci3020744. PMID: 24961422; PMCID: PMC4061861.
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Brain Sci. 2013 May 03;3(2):744-56. doi: 10.3390/brainsci3020744.

Differential Effects of Chronic and Chronic-Intermittent Ethanol Treatment and Its Withdrawal on the Expression of miRNAs.

Brain sciences

Gretchen van Steenwyk, Paulina Janeczek, Joanne M Lewohl

Affiliations

  1. Griffith Health Institute, School of Medical Sciences, Griffith University, Gold Coast Campus, Southport QLD 4215, Australia. [email protected].
  2. Griffith Health Institute, School of Medical Sciences, Griffith University, Gold Coast Campus, Southport QLD 4215, Australia. [email protected].
  3. Griffith Health Institute, School of Medical Sciences, Griffith University, Gold Coast Campus, Southport QLD 4215, Australia. [email protected].

PMID: 24961422 PMCID: PMC4061861 DOI: 10.3390/brainsci3020744

Abstract

Chronic and excessive alcohol misuse results in changes in the expression of selected miRNAs and their mRNA targets in specific regions of the human brain. These expression changes likely underlie the cellular adaptations to long term alcohol misuse. In order to delineate the mechanism by which these expression changes occur, we have measured the expression of six miRNAs including miR-7, miR-153, miR-152, miR-15B, miR-203 and miR-144 in HEK293T, SH SY5Y and 1321 N1 cells following exposure to ethanol. These miRNAs are predicted to target key genes involved in the pathophysiology of alcoholism. Chronic and chronic-intermittent exposure to ethanol, and its removal, resulted in specific changes in miRNA expression in each cell line suggesting that different expression patterns can be elicited with different exposure paradigms and that the mechanism of ethanol's effects is dependent on cell type. Specifically, chronic exposure to ethanol for five days followed by a five day withdrawal period resulted in up-regulation of several miRNAs in each of these cell lines similar to expression changes identified in post mortem human brain. Thus, this model can be used to elucidate the role of miRNAs in regulating gene expression changes that occur in response to ethanol exposure.

References

  1. Alcohol Clin Exp Res. 2000 Dec;24(12):1873-82 - PubMed
  2. Nat Genet. 2000 Nov;26(3):277-81 - PubMed
  3. Alcohol Clin Exp Res. 2012 Jun;36(6):1058-66 - PubMed
  4. Acta Neuropathol. 1989;79(2):200-4 - PubMed
  5. Neurochem Res. 2005 Dec;30(12):1579-88 - PubMed
  6. Brain Res. 1997 Mar 14;751(1):102-12 - PubMed
  7. Mol Pharmacol. 2005 Jun;67(6):2126-36 - PubMed
  8. Alcohol Clin Exp Res. 2010 Apr;34(4):575-87 - PubMed
  9. J Biol Chem. 2011 Aug 5;286(31):27698-705 - PubMed
  10. Alcohol Clin Exp Res. 2011 Nov;35(11):1928-37 - PubMed
  11. J Neurochem. 2004 Sep;90(5):1050-8 - PubMed
  12. Alcohol Res Health. 2002;26(3):219-32 - PubMed
  13. Mol Pharmacol. 2003 Jan;63(1):53-64 - PubMed
  14. Alcohol Clin Exp Res. 2008 Feb;32(2):355-64 - PubMed
  15. Addict Biol. 2014 May;19(3):509-15 - PubMed
  16. Neuron. 2008 Jul 31;59(2):274-87 - PubMed
  17. Science. 1997 Oct 3;278(5335):58-63 - PubMed
  18. Alcohol Clin Exp Res. 2011 Jun;35(6):1041-9 - PubMed
  19. Alcohol Clin Exp Res. 2007 Sep;31(9):1460-6 - PubMed
  20. J Neurosci. 2007 Aug 8;27(32):8546-57 - PubMed
  21. FASEB J. 2002 Jun;16(8):869-71 - PubMed
  22. J Neurochem. 2002 May;81(4):802-13 - PubMed
  23. Methods. 2001 Dec;25(4):402-8 - PubMed
  24. Mol Pharmacol. 2007 Jul;72(1):95-102 - PubMed
  25. Oncogene. 2010 Apr 15;29(15):2302-8 - PubMed
  26. J Pharmacol Toxicol Methods. 2005 May-Jun;51(3):187-200 - PubMed
  27. Neuropsychopharmacology. 2006 Jul;31(7):1574-82 - PubMed
  28. Brain Res. 2010 Jun 22;1340:1-9 - PubMed

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