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Khoo LT, Abas F, Abdullah JO, et al. Anticoagulant Activity of Polyphenolic-Polysaccharides Isolated from Melastoma malabathricum L. Evid Based Complement Alternat Med. 2014;2014:614273doi: 10.1155/2014/614273.
Khoo, L. T., Abas, F., Abdullah, J. O., Mohd Tohit, E. R., & Hamid, M. (2014). Anticoagulant Activity of Polyphenolic-Polysaccharides Isolated from Melastoma malabathricum L. Evidence-based complementary and alternative medicine : eCAM, 2014614273. https://doi.org/10.1155/2014/614273
Khoo, Li Teng, et al. "Anticoagulant Activity of Polyphenolic-Polysaccharides Isolated from Melastoma malabathricum L." Evidence-based complementary and alternative medicine : eCAM vol. 2014 (2014): 614273. doi: https://doi.org/10.1155/2014/614273
Khoo LT, Abas F, Abdullah JO, Mohd Tohit ER, Hamid M. Anticoagulant Activity of Polyphenolic-Polysaccharides Isolated from Melastoma malabathricum L. Evid Based Complement Alternat Med. 2014;2014:614273. doi: 10.1155/2014/614273. Epub 2014 May 20. PMID: 24987430; PMCID: PMC4054800.
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Evid Based Complement Alternat Med. 2014;2014:614273. doi: 10.1155/2014/614273. Epub 2014 May 20.

Anticoagulant Activity of Polyphenolic-Polysaccharides Isolated from Melastoma malabathricum L.

Evidence-based complementary and alternative medicine : eCAM

Li Teng Khoo, Faridah Abas, Janna Ong Abdullah, Eusni Rahayu Mohd Tohit, Muhajir Hamid

Affiliations

  1. Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  2. Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia ; Institute of Biosciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  3. Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  4. Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia ; Institute of Biosciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

PMID: 24987430 PMCID: PMC4054800 DOI: 10.1155/2014/614273

Abstract

Melastoma malabathricum Linn. is a perennial traditional medicine plants that grows abundantly throughout Asian countries. In this study, M. malabathricum Linn. leaf hot water crude extract with anticoagulant activity was purified through solid phase extraction cartridge and examined for the bioactive chemical constituents on blood coagulation reaction. The SPE purified fractions were, respectively, designated as F1, F2, F3, and F4, and each was subjected to the activated partial thromboplastin time (APTT) anticoagulant assay. Active anticoagulant fractions (F1, F2, and F3) were subjected to chemical characterisation evaluation. Besides, neutral sugar for carbohydrate part was also examined. F1, F2, and F3 were found to significantly prolong the anticoagulant activities in the following order, F1 > F2 > F3, in a dose dependent manner. In addition, carbohydrate, hexuronic acid, and polyphenolic moiety were measured for the active anticoagulant fractions (F1, F2, and F3). The characterisation of chemical constituents revealed that all these three fractions contained acidic polysaccharides (rhamnogalacturonan, homogalacturonan, and rhamnose hexose-pectic type polysaccharide) and polyphenolics. Hence, it was concluded that the presence of high hexuronic acids and polysaccharides, as well as polyphenolics in traditional medicinal plant, M. malabathricum, played a role in prolonging blood clotting in the intrinsic pathway.

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