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Kadiyala M, Ponnusankar S, Elango K. Screening of siddha medicinal plants for its in-vitro acetylcholinesterase and butyrylcholinesterase inhibitory activity. Pharmacogn Mag. 2014;10:S294-8doi: 10.4103/0973-1296.133281.
Kadiyala, M., Ponnusankar, S., & Elango, K. (2014). Screening of siddha medicinal plants for its in-vitro acetylcholinesterase and butyrylcholinesterase inhibitory activity. Pharmacognosy magazine, 10S294-8. https://doi.org/10.4103/0973-1296.133281
Kadiyala, Madhuri, et al. "Screening of siddha medicinal plants for its in-vitro acetylcholinesterase and butyrylcholinesterase inhibitory activity." Pharmacognosy magazine vol. 10 (2014): S294-8. doi: https://doi.org/10.4103/0973-1296.133281
Kadiyala M, Ponnusankar S, Elango K. Screening of siddha medicinal plants for its in-vitro acetylcholinesterase and butyrylcholinesterase inhibitory activity. Pharmacogn Mag. 2014 Apr;10:S294-8. doi: 10.4103/0973-1296.133281. PMID: 24991106; PMCID: PMC4078355.
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Pharmacogn Mag. 2014 Apr;10:S294-8. doi: 10.4103/0973-1296.133281.

Screening of siddha medicinal plants for its in-vitro acetylcholinesterase and butyrylcholinesterase inhibitory activity.

Pharmacognosy magazine

Madhuri Kadiyala, Sivasankaran Ponnusankar, Kannan Elango

Affiliations

  1. Department of Pharmacology, JSS College of Pharmacy, Udhagamandalam, The Nilgiris, Tamil Nadu, India.
  2. Department of Pharmacy Practice, JSS College of Pharmacy, Udhagamandalam, The Nilgiris, Tamil Nadu, India.

PMID: 24991106 PMCID: PMC4078355 DOI: 10.4103/0973-1296.133281

Abstract

BACKGROUND: The plants selected for the study were traditionally used in siddha system of medicine in neurological disorders.

AIM: The aim of the following study isto screen the plant species for both acetylcholinesterase (AchE) and butyrylcholinesterase (BuchE) inhibition by in-vitro Ellman's method and a thin layer chromatography bioautographic assay for newer drug candidates for the treatment of Alzheimer's disease.

MATERIALS AND METHODS: Ellman's colorimetric method was performed in a 96 well micro plate for cholinesterases inhibition using galantamine as standard drug.

RESULTS: Present studies confirmed that out of all the tested extracts Hemidesmus indicus R.Br (HI) showed considerable IC50 values for AchE (28.40 ± 0.92 μg/mL) and BuchE (43.47 ± 0.64 μg/mL) inhibition which indicates that HI extract has considerable specificity toward AchE and BuchE compared with all the tested extracts and the activity was followed by Vernonia anthelmintica (VA) Willd and Saussurea lappa Clarke (SL). The bioautograms also confirmed the activity potent extracts.

CONCLUSION: Besides various bioactivities HI, VA and SL exhibited considerable cholinesterases inhibition making it to consider these species for further investigation of new compounds.

Keywords: Acetyl cholinesterase; Alzheimer's disease; Hemidesmus indicus R.Br; butyrylcholinesterase; siddha system of medicine

References

  1. Int Psychogeriatr. 2002;14 Suppl 1:77-91 - PubMed
  2. Toxicol Appl Pharmacol. 2008 Feb 15;227(1):125-35 - PubMed
  3. Lancet. 2002 Apr 13;359(9314):1283-90 - PubMed
  4. Biochem Pharmacol. 1961 Jul;7:88-95 - PubMed
  5. Brain. 1977 Sep;100(3):427-53 - PubMed
  6. Phytochem Anal. 2003 May-Jun;14(3):127-31 - PubMed
  7. J Ethnopharmacol. 2005 Nov 14;102(2):298-301 - PubMed
  8. Pharmacoeconomics. 2000 Sep;18(3):215-23 - PubMed
  9. J Med Chem. 2004 Dec 2;47(25):6248-54 - PubMed
  10. Mol Cells. 2001 Apr 30;11(2):137-43 - PubMed
  11. Fitoterapia. 2006 Sep;77(6):429-34 - PubMed
  12. Fitoterapia. 2003 Feb;74(1-2):184-7 - PubMed
  13. Phytomedicine. 2007 Apr;14(4):289-300 - PubMed

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