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Dianat M, Esmaeilizadeh M, Badavi M, et al. Protective Effects of Crocin on Ischemia-reperfusion Induced Oxidative Stress in Comparison With Vitamin E in Isolated Rat Hearts. Jundishapur J Nat Pharm Prod. 2014;9(2):e17187doi: 10.17795/jjnpp-17187.
Dianat, M., Esmaeilizadeh, M., Badavi, M., Samarbaf-Zadeh, A. R., & Naghizadeh, B. (2014). Protective Effects of Crocin on Ischemia-reperfusion Induced Oxidative Stress in Comparison With Vitamin E in Isolated Rat Hearts. Jundishapur journal of natural pharmaceutical products, 9(2), e17187. https://doi.org/10.17795/jjnpp-17187
Dianat, Mahin, et al. "Protective Effects of Crocin on Ischemia-reperfusion Induced Oxidative Stress in Comparison With Vitamin E in Isolated Rat Hearts." Jundishapur journal of natural pharmaceutical products vol. 9,2 (2014): e17187. doi: https://doi.org/10.17795/jjnpp-17187
Dianat M, Esmaeilizadeh M, Badavi M, Samarbaf-Zadeh AR, Naghizadeh B. Protective Effects of Crocin on Ischemia-reperfusion Induced Oxidative Stress in Comparison With Vitamin E in Isolated Rat Hearts. Jundishapur J Nat Pharm Prod. 2014 Apr 06;9(2):e17187. doi: 10.17795/jjnpp-17187. eCollection 2014 May. PMID: 24872945; PMCID: PMC4036380.
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Jundishapur J Nat Pharm Prod. 2014 Apr 06;9(2):e17187. doi: 10.17795/jjnpp-17187. eCollection 2014 May.

Protective Effects of Crocin on Ischemia-reperfusion Induced Oxidative Stress in Comparison With Vitamin E in Isolated Rat Hearts.

Jundishapur journal of natural pharmaceutical products

Mahin Dianat, Mahdi Esmaeilizadeh, Mohammad Badavi, Ali Reza Samarbaf-Zadeh, Bahareh Naghizadeh

Affiliations

  1. Department of Physiology, Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
  2. Department of Physiology, Diabetic Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
  3. Department of Virology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
  4. Department of Pharmacology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.

PMID: 24872945 PMCID: PMC4036380 DOI: 10.17795/jjnpp-17187

Abstract

BACKGROUND: Myocardial Injury caused by ischemia-reperfusion leads to cardiac dysfunction, tissue injury and metabolic changes. The production of reactive oxygen species (ROS) and lipid peroxidation are accompanied by ischemia-reperfusion injury.

OBJECTIVES: The aim of this study was to assess the cardio protective potential effects of crocin in comparison with vitamin E on antioxidant capacity in ischemia-reperfusion of isolated rat hearts.

MATERIALS AND METHODS: Seventy male Sprague-Dawley rats were randomly divided into seven groups, including: sham, control and experimental groups treated with different doses of crocin (10, 20 and 40 mg/kg) or vitamin E (100 mg/kg) and a combination of crocin (40 mg/kg) with vitamin E (100 mg/kg) that were administrated orally for 21 days. The heart was quickly excised, transferred to a Langendorff apparatus at constant pressure and subjected to 30 minutes of global ischemia followed by 60 minutes of reperfusion. Cardiac damage markers and antioxidant enzymes were measured.

RESULTS: The results showed that superoxide dismutase and catalase enzyme activities increased and Mallon de aldehyde (MDA) decreased in animals pretreated by crocin (40 mg/kg) and vitamin E (100 mg/kg). Moreover, there was a significant improvement in post ischemic recovery of antioxidant capacity during reperfusion in rats receiving a combination of crocin (40 mg/kg) and vitamin E (100 mg/kg).

CONCLUSIONS: The results demonstrated the protective role of crocin on antioxidant capacity, which may partially be related to stability or amplification of antioxidant systems. Like vitamin E, crocin may be beneficial for prevention or treatment of cardiac dysfunction and myocardial infarction in patients with ischemic heart disease.

Keywords: Antioxidants; Crocin; Ischemia-Reperfusion Injury; Rats; Vitamin E

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