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Shamsizadeh A, Soliemani N, Mohammad-Zadeh M, et al. Permanent lesion in rostral ventromedial medulla potentiates swim stress-induced analgesia in formalin test. Iran J Basic Med Sci. 2014;17(3):209-15
Shamsizadeh, A., Soliemani, N., Mohammad-Zadeh, M., & Azhdari-Zarmehri, H. (2014). Permanent lesion in rostral ventromedial medulla potentiates swim stress-induced analgesia in formalin test. Iranian journal of basic medical sciences, 17(3), 209-15.
Shamsizadeh, Ali, et al. "Permanent lesion in rostral ventromedial medulla potentiates swim stress-induced analgesia in formalin test." Iranian journal of basic medical sciences vol. 17,3 (2014): 209-15.
Shamsizadeh A, Soliemani N, Mohammad-Zadeh M, Azhdari-Zarmehri H. Permanent lesion in rostral ventromedial medulla potentiates swim stress-induced analgesia in formalin test. Iran J Basic Med Sci. 2014 Mar;17(3):209-15. PMID: 24847424; PMCID: PMC4016692.
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Iran J Basic Med Sci. 2014 Mar;17(3):209-15.

Permanent lesion in rostral ventromedial medulla potentiates swim stress-induced analgesia in formalin test.

Iranian journal of basic medical sciences

Ali Shamsizadeh, Neda Soliemani, Mohammad Mohammad-Zadeh, Hassan Azhdari-Zarmehri

Affiliations

  1. Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
  2. Department of Physiology & Pharmacology, Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran.
  3. Department of Basic Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.

PMID: 24847424 PMCID: PMC4016692

Abstract

OBJECTIVE(S): There are many reports about the role of rostral ventromedial medulla (RVM) in modulating stress-induced analgesia (SIA). In the previous study we demonstrated that temporal inactivation of RVM by lidocaine potentiated stress-induced analgesia. In this study, we investigated the effect of permanent lesion of the RVM on SIA by using formalin test as a model of acute inflammatory pain.

MATERIALS AND METHODS: Three sets of experiments were conducted: (1) Application of stress protocol (2) Formalin injection after exposing the animals to the swim stress (3) Either the relevant vehicle or dopamine receptor 1 (D1) agonist R-SKF38393 was injected into the RVM to cause a lesion. For permanent lesion of RVM, R-SKF38393 was injected into the RVM. Forced swim stress in water was employed in adult male rats. Nociceptive responses were measured by formalin test (50µl injection of formalin 2% subcutaneously into hind paw) and pain related behaviors were monitored for 90 min.

RESULTS: In the unstressed rats, permanent lesion of the RVM by R-SKF38393 decreased formalin-induced nociceptive behaviors in phase 1, while in stressed rats, injection of R-SKF38393 into the RVM potentiated swim stress-induced antinociception in phase 1 and interphase, phase 2A of formalin test. Furthermore, R-SKF38393 had pronociceptive effects in phase2B whereas injections of R-SKF38393 resulted in significant difference in nociceptive bahaviours in all phases of formalin test (P<0.05).

CONCLUSION: The result of the present study demonstrated that permanent inactivation of RVM can potentiate stress-induced analgesia in formalin test.

Keywords: Analgesia; Formalin test; Inactivation; Lesion; Rostral ventromedial medulla; Swim stress

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