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Ahn JY, Kim IY, Oh SJ, et al. Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Lab Anim Res. 2014;30(2):45-53doi: 10.5625/lar.2014.30.2.45.
Ahn, J. Y., Kim, I. Y., Oh, S. J., Hwang, H. S., Yi, S. S., Kim, Y. N., Shin, J. H., Yoon, Y. S., & Seong, J. K. (2014). Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Laboratory animal research, 30(2), 45-53. https://doi.org/10.5625/lar.2014.30.2.45
Ahn, Ji Ye, et al. "Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry." Laboratory animal research vol. 30,2 (2014): 45-53. doi: https://doi.org/10.5625/lar.2014.30.2.45
Ahn JY, Kim IY, Oh SJ, Hwang HS, Yi SS, Kim YN, Shin JH, Yoon YS, Seong JK. Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Lab Anim Res. 2014 Jun;30(2):45-53. doi: 10.5625/lar.2014.30.2.45. Epub 2014 Jun 23. PMID: 24999358; PMCID: PMC4079831.
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Lab Anim Res. 2014 Jun;30(2):45-53. doi: 10.5625/lar.2014.30.2.45. Epub 2014 Jun 23.

Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry.

Laboratory animal research

Ji Ye Ahn, Il Yong Kim, Sae Jin Oh, Hye Sook Hwang, Sun Shin Yi, Yo Na Kim, Jae Hoon Shin, Yeo Sung Yoon, Je Kyung Seong

Affiliations

  1. Laboratory of Developmental Biology and Genetics, College of Veterinary Medicine, BK21Plus Program for Creative Veterinary Science Research, BIO-MAX institute, Seoul National University, Seoul, Korea.
  2. Department of Biomedical Laboratory Science, College of Biomedical Science, Soonchunhyang University, Asan, Korea.

PMID: 24999358 PMCID: PMC4079831 DOI: 10.5625/lar.2014.30.2.45

Abstract

Pig pancreas may be a therapeutic resource for human diabetic patients. However, this potential is hindered by a lack of knowledge of the molecular events of pig pancreas development. In this study, the embryonic day 60, neonate and 6-month protein profiles of pig pancreas were ascertained at using two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry. Twenty four proteins were differentially expressed during pig pancreas development. Among them, 12 spots increased and 7 spots decreased according to development. The expression of 5 protein were highest at birth. Expression of digestive enzymes including trypsin, pancreatic triacylglycerol lipase and pancreatic alpha-amylase was elevated in adults, whereas chymotrypsins were highly expressed in neonates. Proteins that were abundantly expressed during gestation were alpha-1-antitrypsin, alpha-fetoprotein and transferrins. Taken together, we found out that several proteins were significantly up- or down- regulated from pig pancreas based on developmental stage. This study will provide basis for understanding development of pig pancreas.

Keywords: 2-DE proteomics; Domestic pig; MALDI-TOF; development; pancreas

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