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Upadhaya SD, Song JY, Park MA, et al. Isolation, screening and identification of Swine gut microbiota with ochratoxin a biodegradation ability. Asian-Australas J Anim Sci. 2012;25(1):114-21doi: 10.5713/ajas.2011.11104.
Upadhaya, S. D., Song, J. Y., Park, M. A., Seo, J. K., Yang, L., Lee, C. H., Cho, K. J., & Ha, J. K. (2012). Isolation, screening and identification of Swine gut microbiota with ochratoxin a biodegradation ability. Asian-Australasian journal of animal sciences, 25(1), 114-21. https://doi.org/10.5713/ajas.2011.11104
Upadhaya, Santi Devi, et al. "Isolation, screening and identification of Swine gut microbiota with ochratoxin a biodegradation ability." Asian-Australasian journal of animal sciences vol. 25,1 (2012): 114-21. doi: https://doi.org/10.5713/ajas.2011.11104
Upadhaya SD, Song JY, Park MA, Seo JK, Yang L, Lee CH, Cho KJ, Ha JK. Isolation, screening and identification of Swine gut microbiota with ochratoxin a biodegradation ability. Asian-Australas J Anim Sci. 2012 Jan;25(1):114-21. doi: 10.5713/ajas.2011.11104. PMID: 25049486; PMCID: PMC4092918.
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Asian-Australas J Anim Sci. 2012 Jan;25(1):114-21. doi: 10.5713/ajas.2011.11104.

Isolation, screening and identification of Swine gut microbiota with ochratoxin a biodegradation ability.

Asian-Australasian journal of animal sciences

Santi Devi Upadhaya, Jae Yong Song, Min Ah Park, Ja Kyeom Seo, Liu Yang, Chan Ho Lee, Kyung J Cho, Jong K Ha

Affiliations

  1. Genebiotech co., Ltd., Gongju, Republic of Korea .

PMID: 25049486 PMCID: PMC4092918 DOI: 10.5713/ajas.2011.11104

Abstract

The potential for ochratoxin A (OTA) degradation by swine intestinal microbiota was assessed in the current study. Intestinal content that was collected aseptically from swine was spiked with 100 ppb OTA and incubated for 6 and 12 h at 39°C. An OTA assay was conducted using the incubated samples, and it was found that 20% of the OTA toxin was detoxified, indicating the presence of microbes capable of OTA degradation. Twenty-eight bacterial species were isolated anaerobically in M 98-5 media and 45 bacterial species were isolated using nutrient broth aerobically. Screening results showed that one anaerobic bacterial isolate, named MM11, detoxified more than 75% of OTA in liquid media. Furthermore, 1.0 ppm OTA was degraded completely after 24 h incubation on a solid 'corn' substrate. The bacterium was identified by 16S rDNA sequencing as having 97% sequence similarity with Eubacterium biforme. The isolation of an OTA-degrading bacterium from the swine natural flora is of great importance for OTA biodegradation and may be a valuable potential source for OTA-degradation enzymes in industrial applications.

Keywords: Degradation; Enzyme-linked Immunosorbent Assay; Isolation; Ochratoxin A; Swine Intestinal Microbiota

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