Asian-Australas J Anim Sci. 2012 Feb;25(2):234-9. doi: 10.5713/ajas.2011.11242. Epub 2012 Feb 01.

The Effect of Forage Level and Oil Supplement on Butyrivibrio fibrisolvens and Anaerovibrio lipolytica in Continuous Culture Fermenters.

Asian-Australasian journal of animal sciences

P Gudla, A Ishlak, A A AbuGhazaleh

PMID: 25049556 PMCID: PMC4093135 DOI: 10.5713/ajas.2011.11242

Abstract

The objective of this study was to evaluate the effects of forage level and oil supplement on selected strains of rumen bacteria believed to be involved in biohydrogenation (BH). A continuous culture system consisting of four fermenters was used in a 4×4 Latin square design with a factorial arrangement of treatments, with four 10 d consecutive periods. Treatment diets were: i) high forage diet (70:30 forage to concentrate (dry matter basis); HFC), ii) high forage plus oil supplement (HFO), iii) low forage diet (30:70 forage to concentrate; LFC), and iv) low forage plus oil supplement (LFO). The oil supplement was a blend of fish oil and soybean oil added at 1 and 2 g/100 g dry matter, respectively. Treatment diets were fed for 10 days and samples were collected from each fermenter on the last day of each period 3 h post morning feeding. The concentrations of vaccenic acid (t11C18:1; VA) and c9t11 conjugated linoleic acid (CLA) were greater with the high forage diet while the concentrations of t10 C18:1 and t10c12 CLA were greater with the low forage diet and addition of oil supplement increased their concentrations at both forage levels. The DNA abundance of Anaerovibrio lipolytica, and Butyrivibrio fibrisolvens vaccenic acid subgroup (Butyrivibrio VA) were lower with the low forage diets but not affected by oil supplement. The DNA abundance of Butyrivibrio fibrisolvens stearic acid producer subgroup (Butyrivibrio SA) was not affected by forage level or oil supplement. In conclusion, oil supplement had no effects on the tested rumen bacteria and forage level affected Anaerovibrio lipolytica and Butyrivibrio VA.

Keywords: Biohydrogenation; Fermenters; Forage Level; Oil; Trans Fatty Acids

References

1. Reprod Nutr Dev. 1996;36(1):53-63 - PubMed
2. J Dairy Sci. 2010 Jul;93(7):3275-86 - PubMed
3. J Dairy Sci. 2009 Mar;92(3):1108-16 - PubMed
4. J Dairy Sci. 2002 Jul;85(7):1764-6 - PubMed
5. Animal. 2007 Jun;1(5):660-5 - PubMed
6. Br J Nutr. 2009 Dec;102(11):1583-9 - PubMed
7. J Dairy Sci. 2009 Sep;92(9):4456-66 - PubMed
8. J Anim Physiol Anim Nutr (Berl). 2011 Jun;95(3):313-9 - PubMed
9. Animal. 2008 Dec;2(12):1859-69 - PubMed
10. Nutr Res. 2010 Mar;30(3):179-85 - PubMed
11. J Nutr. 2008 May;138(5):889-96 - PubMed
12. J Dairy Sci. 2004 Aug;87(8):2472-85 - PubMed
13. J Nutr Biochem. 2006 Dec;17(12):789-810 - PubMed
14. J Anim Sci. 2010 May;88(5):1694-705 - PubMed
15. Antonie Van Leeuwenhoek. 2007 May;91(4):303-14 - PubMed
16. J Nutr. 2002 Jun;132(6):1235-41 - PubMed
17. Lipids. 2009 Jan;44(1):53-62 - PubMed
18. J Gen Microbiol. 1965 Feb;38:167-80 - PubMed
19. FEMS Microbiol Ecol. 2010 Aug;73(2):396-407 - PubMed
20. J Anim Sci. 2010 Aug;88(8):2684-91 - PubMed
21. BMC Microbiol. 2010 Feb 18;10:52 - PubMed
22. Appl Environ Microbiol. 2001 Jun;67(6):2766-74 - PubMed
23. J Microbiol. 2011 Apr;49(2):216-23 - PubMed
24. J Anim Sci. 2003 Dec;81(12):3174-81 - PubMed
25. J Nutr. 2007 Dec;137(12):2599-607 - PubMed
26. Res Vet Sci. 2008 Apr;84(2):215-24 - PubMed
27. Lipids. 2009 May;44(5):449-58 - PubMed
28. Antonie Van Leeuwenhoek. 2007 May;91(4):417-22 - PubMed

Publication Types

• Journal Article