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Sommer YL, Verdon CP, Fresquez MR, et al. Measurement of mercury species in human blood using triple spike isotope dilution with SPME-GC-ICP-DRC-MS. Anal Bioanal Chem. 2014;406(20):5039-47doi: 10.1007/s00216-014-7907-4.
Sommer, Y. L., Verdon, C. P., Fresquez, M. R., Ward, C. D., Wood, E. B., Pan, Y., Caldwell, K. L., & Jones, R. L. (2014). Measurement of mercury species in human blood using triple spike isotope dilution with SPME-GC-ICP-DRC-MS. Analytical and bioanalytical chemistry, 406(20), 5039-47. https://doi.org/10.1007/s00216-014-7907-4
Sommer, Yuliya L, et al. "Measurement of mercury species in human blood using triple spike isotope dilution with SPME-GC-ICP-DRC-MS." Analytical and bioanalytical chemistry vol. 406,20 (2014): 5039-47. doi: https://doi.org/10.1007/s00216-014-7907-4
Sommer YL, Verdon CP, Fresquez MR, Ward CD, Wood EB, Pan Y, Caldwell KL, Jones RL. Measurement of mercury species in human blood using triple spike isotope dilution with SPME-GC-ICP-DRC-MS. Anal Bioanal Chem. 2014 Aug;406(20):5039-47. doi: 10.1007/s00216-014-7907-4. Epub 2014 Jun 20. PMID: 24948088; PMCID: PMC4685456.
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Anal Bioanal Chem. 2014 Aug;406(20):5039-47. doi: 10.1007/s00216-014-7907-4. Epub 2014 Jun 20.

Measurement of mercury species in human blood using triple spike isotope dilution with SPME-GC-ICP-DRC-MS.

Analytical and bioanalytical chemistry

Yuliya L Sommer, Carl P Verdon, Mark R Fresquez, Cynthia D Ward, Elliott B Wood, Yi Pan, Kathleen L Caldwell, Robert L Jones

Affiliations

  1. Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Analytical Sciences, Inorganic and Radiation Analytical Toxicology Branch, 4770 Buford Highway, N.E., MS F-50, Atlanta, GA, 30341, USA, [email protected].

PMID: 24948088 PMCID: PMC4685456 DOI: 10.1007/s00216-014-7907-4

Abstract

The measurement of different mercury compounds in human blood can provide valuable information about the type of mercury exposure. To this end, our laboratory developed a biomonitoring method for the quantification of inorganic (iHg), methyl (MeHg), and ethyl (EtHg) mercury in whole blood using a triple-spike isotope dilution (TSID) quantification method employing capillary gas chromatography (GC) and inductively coupled dynamic reaction cell mass spectrometry (ICP-DRC-MS). We used a robotic CombiPAL(®) sample handling station featuring twin fiber-based solid-phase microextraction (SPME) injector heads. The use of two SPME fibers significantly reduces sample analysis cycle times making this method very suitable for high sample throughput, which is a requirement for large public health biomonitoring studies. Our sample preparation procedure involved solubilization of blood samples with tetramethylammonium hydroxide (TMAH) followed by the derivatization with sodium tetra(n-propyl)borate (NaBPr(4)) to promote volatility of mercury species. We thoroughly investigated mercury species stability in the blood matrix during the course of sample treatment and analysis. The method accuracy for quantifying iHg, MeHg, and EtHg was validated using NIST standard reference materials (SRM 955c level 3) and the Centre de Toxicologie du Québec (CTQ) proficiency testing (PT) samples. The limit of detection (LOD) for iHg, MeHg, and EtHg in human blood was determined to be 0.27, 0.12, and 0.16 μg/L, respectively.

References

  1. Anal Chem. 2009 Aug 15;81(16):6774-8 - PubMed
  2. Anal Bioanal Chem. 2009 May;394(1):199-205 - PubMed
  3. Anal Chem. 2009 Jun 15;81(12):5075-9 - PubMed
  4. Int J Hyg Environ Health. 2009 Nov;212(6):588-98 - PubMed
  5. J Chromatogr A. 2004 Jan 30;1025(1):77-84 - PubMed
  6. Anal Bioanal Chem. 2008 Apr;390(8):2123-32 - PubMed
  7. Talanta. 2003 Jul 4;60(4):821-30 - PubMed

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