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Armingohar Z, Jørgensen JJ, Kristoffersen AK, et al. Bacteria and bacterial DNA in atherosclerotic plaque and aneurysmal wall biopsies from patients with and without periodontitis. J Oral Microbiol. 2014;6doi: 10.3402/jom.v6.23408.
Armingohar, Z., Jørgensen, J. J., Kristoffersen, A. K., Abesha-Belay, E., & Olsen, I. (2014). Bacteria and bacterial DNA in atherosclerotic plaque and aneurysmal wall biopsies from patients with and without periodontitis. Journal of oral microbiology, 6. https://doi.org/10.3402/jom.v6.23408
Armingohar, Zahra, et al. "Bacteria and bacterial DNA in atherosclerotic plaque and aneurysmal wall biopsies from patients with and without periodontitis." Journal of oral microbiology vol. 6 (2014). doi: https://doi.org/10.3402/jom.v6.23408
Armingohar Z, Jørgensen JJ, Kristoffersen AK, Abesha-Belay E, Olsen I. Bacteria and bacterial DNA in atherosclerotic plaque and aneurysmal wall biopsies from patients with and without periodontitis. J Oral Microbiol. 2014 May 15;6. doi: 10.3402/jom.v6.23408. eCollection 2014. PMID: 25006361; PMCID: PMC4024159.
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J Oral Microbiol. 2014 May 15;6. doi: 10.3402/jom.v6.23408. eCollection 2014.

Bacteria and bacterial DNA in atherosclerotic plaque and aneurysmal wall biopsies from patients with and without periodontitis.

Journal of oral microbiology

Zahra Armingohar, Jørgen J Jørgensen, Anne Karin Kristoffersen, Emnet Abesha-Belay, Ingar Olsen

Affiliations

  1. Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway.
  2. Department of Vascular Surgery, Oslo University Hospital, Aker and University of Oslo, Oslo, Norway.

PMID: 25006361 PMCID: PMC4024159 DOI: 10.3402/jom.v6.23408

Abstract

BACKGROUND: Several studies have reported an association between chronic periodontitis (CP) and cardiovascular diseases. Detection of periodontopathogens, including red complex bacteria (RCB), in vascular lesions has suggested these bacteria to be involved in the pathogenesis of atherosclerosis and abdominal aortic aneurysms.

OBJECTIVE: In this study, we investigate bacteria and their DNA in vascular biopsies from patients with vascular diseases (VD; i.e. abdominal aortic aneurysms, atherosclerotic carotid, and common femoral arteries), with and without CP.

METHODS: DNA was extracted from vascular biopsies selected from 40 VD patients: 30 with CP and 10 without CP. The V3-V5 region of the 16S rDNA (V3-V5) was polymerase chain reaction (PCR)-amplified, and the amplicons were cloned into Escherichia coli, sequenced, and classified (GenBank and the Human Oral Microbiome database). Species-specific primers were used for the detection of Porphyromonas gingivalis. In addition, 10 randomly selected vascular biopsies from the CP group were subjected to scanning electron microscopy (SEM) for visualization of bacteria. Checkerboard DNA-DNA hybridization was performed to assess the presence of RCB in 10 randomly selected subgingival plaque samples from CP patients.

RESULTS: A higher load and mean diversity of bacteria were detected in vascular biopsies from VD patients with CP compared to those without CP. Enterobacteriaceae were frequently detected in vascular biopsies together with cultivable, commensal oral, and not-yet-cultured bacterial species. While 70% of the subgingival plaque samples from CP patients showed presence of RCB, only P. gingivalis was detected in one vascular biopsy. Bacterial cells were seen in all 10 vascular biopsies examined by SEM.

CONCLUSIONS: A higher bacterial load and more diverse colonization were detected in VD lesions of CP patients as compared to patients without CP. This indicated that a multitude of bacterial species both from the gut and the oral cavity, rather than exclusively periodontopathogens, may be involved as additional risk factors in the pathogenesis of VD.

Keywords: 16S rDNA; Porphyromonas gingivalis; chronic periodontitis; vascular disease

References

  1. Appl Environ Microbiol. 2009 Dec;75(23):7537-41 - PubMed
  2. J Pharmacol Sci. 2010;113(2):120-5 - PubMed
  3. Oral Microbiol Immunol. 1999 Jun;14(3):137-42 - PubMed
  4. N Engl J Med. 1999 Jan 14;340(2):115-26 - PubMed
  5. Mol Biol Evol. 2011 Oct;28(10):2731-9 - PubMed
  6. Circulation. 2006 Feb 21;113(7):929-37 - PubMed
  7. J Clin Microbiol. 2001 Mar;39(3):1114-7 - PubMed
  8. Arch Oral Biol. 2011 Sep;56(9):899-906 - PubMed
  9. J Bacteriol. 2010 Oct;192(19):5002-17 - PubMed
  10. Clin Microbiol Rev. 1996 Apr;9(2):148-65 - PubMed
  11. Oral Microbiol Immunol. 2004 Dec;19(6):352-62 - PubMed
  12. Minerva Stomatol. 2007 Apr;56(4):169-79 - PubMed
  13. Periodontol 2000. 2004;34:9-21 - PubMed
  14. J Bacteriol. 2001 Jun;183(12):3770-83 - PubMed
  15. Microbes Infect. 2006 Mar;8(3):687-93 - PubMed
  16. Infect Disord Drug Targets. 2010 Apr;10(2):84-90 - PubMed
  17. Oral Dis. 2008 Mar;14(2):95-101 - PubMed
  18. Oral Microbiol Immunol. 1990 Jun;5(3):149-54 - PubMed
  19. Chin Med J (Engl). 2008 Aug 20;121(16):1595-7 - PubMed
  20. J Periodontol. 2002 Aug;73(8):868-70 - PubMed
  21. J Exp Med. 2006 Jun 12;203(6):1591-601 - PubMed
  22. J Clin Periodontol. 2012 Mar;39(3):213-28 - PubMed
  23. Shock. 2009 Oct;32(4):374-8 - PubMed
  24. J Periodontal Res. 2006 Aug;41(4):350-3 - PubMed
  25. Future Cardiol. 2012 Jan;8(1):123-38 - PubMed
  26. Oral Microbiol Immunol. 2008 Apr;23(2):170-2 - PubMed
  27. J Vasc Surg. 2003 Dec;38(6):1384-9 - PubMed
  28. Shock. 2003 Mar;19(3):229-37 - PubMed
  29. J Clin Periodontol. 2008 Jun;35(6):473-8 - PubMed
  30. Am Fam Physician. 2012 May 15;85(10):981-6 - PubMed
  31. Eur J Vasc Endovasc Surg. 2008 Feb;35(2):153-8 - PubMed
  32. J Clin Periodontol. 2013 Apr;40 Suppl 14:S24-9 - PubMed
  33. J Periodontol. 2008 Aug;79(8 Suppl):1560-8 - PubMed
  34. J Clin Periodontol. 2013 Apr;40 Suppl 14:S30-50 - PubMed
  35. Eur J Clin Invest. 2005 Jan;35(1):13-6 - PubMed
  36. Atherosclerosis. 2008 Nov;201(1):192-7 - PubMed
  37. Am J Physiol Gastrointest Liver Physiol. 2003 Sep;285(3):G621-9 - PubMed
  38. J Periodontol. 2007 Sep;78(9):1718-23 - PubMed
  39. J Am Heart Assoc. 2013 Oct 28;2(6):e000254 - PubMed
  40. J Clin Microbiol. 1999 May;37(5):1426-30 - PubMed
  41. Infect Dis Clin North Am. 2002 Jun;16(2):297-318 - PubMed
  42. FEMS Microbiol Lett. 2000 Jun 15;187(2):139-44 - PubMed
  43. J Vasc Surg. 2006 Nov;44(5):1055-60 - PubMed
  44. J Intern Med. 2011 Sep;270(3):273-80 - PubMed
  45. J Dent Res. 2010 Sep;89(9):879-902 - PubMed
  46. J Clin Microbiol. 2000 May;38(5):1909-14 - PubMed
  47. Oral Microbiol Immunol. 2006 Feb;21(1):53-60 - PubMed
  48. J Clin Microbiol. 2006 Sep;44(9):3313-7 - PubMed
  49. Arterioscler Thromb Vasc Biol. 2010 Jun;30(6):1075-7 - PubMed
  50. J Microbiol Methods. 2009 Mar;76(3):217-25 - PubMed
  51. J Med Microbiol. 2005 Jan;54(Pt 1):93-96 - PubMed
  52. J Clin Periodontol. 2006 Jun;33(6):401-7 - PubMed
  53. Arterioscler Thromb Vasc Biol. 2005 Mar;25(3):e17-8 - PubMed
  54. J Dent Res. 2012 Mar;91(3):255-60 - PubMed
  55. J Periodontol. 2005 May;76(5):731-6 - PubMed
  56. J Microbiol Methods. 2003 Dec;55(3):541-55 - PubMed
  57. J Med Microbiol. 2002 Dec;51(12):1090-1096 - PubMed
  58. Scand J Infect Dis. 2010 Jul;42(6-7):405-11 - PubMed
  59. J Clin Microbiol. 2000 Oct;38(10):3623-30 - PubMed
  60. Anaerobe. 1997 Aug;3(4):237-43 - PubMed
  61. J Periodontal Res. 2004 Dec;39(6):442-6 - PubMed
  62. Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4592-8 - PubMed
  63. J Med Microbiol. 2009 Dec;58(Pt 12):1568-1575 - PubMed
  64. J Clin Periodontol. 2009 Jul;36 Suppl 10:15-9 - PubMed
  65. Database (Oxford). 2010 Jul 06;2010:baq013 - PubMed
  66. Bioinformatics. 2011 Aug 15;27(16):2194-200 - PubMed
  67. PLoS One. 2011;6(8):e22674 - PubMed

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