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Azeemuddin M, Viswanatha GL, Rafiq M, et al. An improved experimental model of hemorrhoids in rats: evaluation of antihemorrhoidal activity of an herbal formulation. ISRN Pharmacol. 2014;2014:530931doi: 10.1155/2014/530931.
Azeemuddin, M., Viswanatha, G. L., Rafiq, M., Thippeswamy, A. H., Baig, M. R., Kavya, K. J., Patki, P. S., & Shyam, R. (2014). An improved experimental model of hemorrhoids in rats: evaluation of antihemorrhoidal activity of an herbal formulation. ISRN pharmacology, 2014530931. https://doi.org/10.1155/2014/530931
Azeemuddin, Mohammed, et al. "An improved experimental model of hemorrhoids in rats: evaluation of antihemorrhoidal activity of an herbal formulation." ISRN pharmacology vol. 2014 (2014): 530931. doi: https://doi.org/10.1155/2014/530931
Azeemuddin M, Viswanatha GL, Rafiq M, Thippeswamy AH, Baig MR, Kavya KJ, Patki PS, Shyam R. An improved experimental model of hemorrhoids in rats: evaluation of antihemorrhoidal activity of an herbal formulation. ISRN Pharmacol. 2014 Mar 11;2014:530931. doi: 10.1155/2014/530931. eCollection 2014. PMID: 25006493; PMCID: PMC3967498.
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ISRN Pharmacol. 2014 Mar 11;2014:530931. doi: 10.1155/2014/530931. eCollection 2014.

An improved experimental model of hemorrhoids in rats: evaluation of antihemorrhoidal activity of an herbal formulation.

ISRN pharmacology

Mohammed Azeemuddin, Gollapalle Lakshminarayanashastry Viswanatha, Mohamed Rafiq, Agadi HireMath Thippeswamy, Mirza Rizwan Baig, Kethaganahalli Jayaramaiah Kavya, Pralhad Sadashiv Patki, Ramakrishnan Shyam

Affiliations

  1. Department of Pharmacology, R&D Center, The Himalaya Drug Company, Makali, Bangalore, Karnataka 562 162, India.

PMID: 25006493 PMCID: PMC3967498 DOI: 10.1155/2014/530931

Abstract

Objective. To improve the existing experimental model of croton oil-induced hemorrhoids in rats by using Evans Blue (EB) dye extravasation technique. Further, an herbal formulation (Pilex) was evaluated for its antihemorrhoidal activity in this model. Methods. Two sets of experiments were carried out: first to improve the experimental model and to validate the same using Pilex and second to evaluate the effect of Pilex on cytoarchitecture of rectoanal tissue in croton oil-induced hemorrhoids. In both sets, hemorrhoids were induced to all the animals, except normal controls, by applying croton oil via rectoanal region and the effect of Pilex ointment (PO), Pilex granules (PG), and combination of PG and PO was evaluated. In the first set, extravasation of EB dye, TNF- α , IL-6, and rectoanal coefficient (RAC) was determined. In the second set, severity of score, RAC, and histopathology were evaluated. Results. The elevated levels of TNF- α , IL-6, and extravasations of EB dye were decreased with the Pilex treatment. The cytoarchitecture of rectoanal portion of the animals treated with Pilex was near to normal. Conclusion. The improved experimental model of hemorrhoid is useful in quantifying the inflammatory exudates and extent of inflammation. In this improved experimental model Pilex showed antihemorrhoidal activity, which further validates its clinical usage.

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