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Na GH, Kim DG, Kim YH, et al. Effects of glucose concentration in the medium on rat hepatocyte culture. Ann Surg Treat Res. 2014;87(2):53-60doi: 10.4174/astr.2014.87.2.53.
Na, G. H., Kim, D. G., Kim, Y. H., Han, J. H., & Jung, E. S. (2014). Effects of glucose concentration in the medium on rat hepatocyte culture. Annals of surgical treatment and research, 87(2), 53-60. https://doi.org/10.4174/astr.2014.87.2.53
Na, Gun Hyung, et al. "Effects of glucose concentration in the medium on rat hepatocyte culture." Annals of surgical treatment and research vol. 87,2 (2014): 53-60. doi: https://doi.org/10.4174/astr.2014.87.2.53
Na GH, Kim DG, Kim YH, Han JH, Jung ES. Effects of glucose concentration in the medium on rat hepatocyte culture. Ann Surg Treat Res. 2014 Aug;87(2):53-60. doi: 10.4174/astr.2014.87.2.53. Epub 2014 Jul 29. PMID: 25114883; PMCID: PMC4127907.
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Ann Surg Treat Res. 2014 Aug;87(2):53-60. doi: 10.4174/astr.2014.87.2.53. Epub 2014 Jul 29.

Effects of glucose concentration in the medium on rat hepatocyte culture.

Annals of surgical treatment and research

Gun Hyung Na, Dong Goo Kim, Young Hui Kim, Jae Hyun Han, Eun Sun Jung

Affiliations

  1. Department of Surgery, Seoul St. Mary's Hospital, The Catholic University of Korea College of Medicine, Seoul, Korea.
  2. Department of Pathology, Seoul St. Mary's Hospital, The Catholic University of Korea College of Medicine, Seoul, Korea.

PMID: 25114883 PMCID: PMC4127907 DOI: 10.4174/astr.2014.87.2.53

Abstract

PURPOSE: To determine the optimum culture conditions by investigating isolated rat hepatocytes cultured in medium containing different glucose concentrations.

METHODS: Hepatocytes were isolated from rats using a two-step perfusion technique and divided into the following two groups cultured in medium containing different glucose concentrations: (1) low-glucose group and (2) high-glucose group. Total cell count and viability of cultured rat hepatocytes and liver function parameters (i.e., concentrations of albumin, ammonia, and urea in the culture medium) were measured. The morphology of cultured rat hepatocytes was examined by staining with hematoxylin and eosin, and albumin receptor expression was confirmed by immunofluorescence.

RESULTS: Total cell count and viability showed smaller increases in the low-glucose group than the high-glucose group, although the difference was not statistically significant (P = 0.112 and P = 0.147, respectively). The levels of albumin (P = 0.943), ammonia (P = 0.744), and urea (P = 0.709) were not significantly different between the two groups. In both groups, the function of cultured hepatocytes decreased significantly over time. The morphology of hepatocytes was well maintained in both groups at 3 days. On day 7, the cytoplasm was transformed into a spindle shape. On day 10, these changes were exaggerated, and were more prominent in the high-glucose group.

CONCLUSION: Morphological assessment indicated that low-glucose culture medium is better than high-glucose culture medium for culturing of hepatocytes, although there was not significantly different in functional assessment. The cultured hepatocytes with low-glucose culture medium could be maintained for 7 days.

Keywords: Cell transplantation; Culture; Glucose; Hepatocytes; Rats

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