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Bueschl C, Kluger B, Lemmens M, et al. A novel stable isotope labelling assisted workflow for improved untargeted LC-HRMS based metabolomics research. Metabolomics. 2013;10(4):754-769doi: 10.1007/s11306-013-0611-0.
Bueschl, C., Kluger, B., Lemmens, M., Adam, G., Wiesenberger, G., Maschietto, V., Marocco, A., Strauss, J., Bödi, S., Thallinger, G. G., Krska, R., & Schuhmacher, R. (2014). A novel stable isotope labelling assisted workflow for improved untargeted LC-HRMS based metabolomics research. Metabolomics : Official journal of the Metabolomic Society, 10(4), 754-769. https://doi.org/10.1007/s11306-013-0611-0
Bueschl, Christoph, et al. "A novel stable isotope labelling assisted workflow for improved untargeted LC-HRMS based metabolomics research." Metabolomics : Official journal of the Metabolomic Society vol. 10,4 (2014): 754-769. doi: https://doi.org/10.1007/s11306-013-0611-0
Bueschl C, Kluger B, Lemmens M, Adam G, Wiesenberger G, Maschietto V, Marocco A, Strauss J, Bödi S, Thallinger GG, Krska R, Schuhmacher R. A novel stable isotope labelling assisted workflow for improved untargeted LC-HRMS based metabolomics research. Metabolomics. 2014;10(4):754-769. doi: 10.1007/s11306-013-0611-0. Epub 2013 Dec 04. PMID: 25057268; PMCID: PMC4098048.
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Metabolomics. 2014;10(4):754-769. doi: 10.1007/s11306-013-0611-0. Epub 2013 Dec 04.

A novel stable isotope labelling assisted workflow for improved untargeted LC-HRMS based metabolomics research.

Metabolomics : Official journal of the Metabolomic Society

Christoph Bueschl, Bernhard Kluger, Marc Lemmens, Gerhard Adam, Gerlinde Wiesenberger, Valentina Maschietto, Adriano Marocco, Joseph Strauss, Stephan Bödi, Gerhard G Thallinger, Rudolf Krska, Rainer Schuhmacher

Affiliations

  1. Department for Agrobiotechnology (IFA-Tulln), Center for Analytical Chemistry and Institute for Biotechnology in Plant Production, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Str. 20, 3430 Tulln, Austria.
  2. Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Str. 24, 3430 Tulln, Austria.
  3. Institute of Agronomy, Genetics and Field Crops, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, Italy.
  4. Health and Environment Department, Bioresources - Fungal Genetics and Genomics, Austrian Institute of Technology (AIT), Konrad-Lorenz-Str. 24, 3430 Tulln, Austria.
  5. Institute for Genomics and Bioinformatics, Graz University of Technology, Petersgasse 14, 8010 Graz, Austria.
  6. Core Facility Bioinformatics, Austrian Centre for Industrial Biotechnology, Petersgasse 14, 8010 Graz, Austria.

PMID: 25057268 PMCID: PMC4098048 DOI: 10.1007/s11306-013-0611-0

Abstract

Many untargeted LC-ESI-HRMS based metabolomics studies are still hampered by the large proportion of non-biological sample derived signals included in the generated raw data. Here, a novel, powerful stable isotope labelling (SIL)-based metabolomics workflow is presented, which facilitates global metabolome extraction, improved metabolite annotation and metabolome wide internal standardisation (IS). The general concept is exemplified with two different cultivation variants, (1) co-cultivation of the plant pathogenic fungi

Keywords: 13C-labelling; Fusarium; Internal standardisation; Maize; Metabolomics; Wheat

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