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Cai Y, Mikkelsen JG. Driving DNA transposition by lentiviral protein transduction. Mob Genet Elements. 2014;4:e29591doi: 10.4161/mge.29591.
Cai, Y., & Mikkelsen, J. G. (2014). Driving DNA transposition by lentiviral protein transduction. Mobile genetic elements, 4e29591. https://doi.org/10.4161/mge.29591
Cai, Yujia, and Mikkelsen, Jacob Giehm. "Driving DNA transposition by lentiviral protein transduction." Mobile genetic elements vol. 4 (2014): e29591. doi: https://doi.org/10.4161/mge.29591
Cai Y, Mikkelsen JG. Driving DNA transposition by lentiviral protein transduction. Mob Genet Elements. 2014 Jun 23;4:e29591. doi: 10.4161/mge.29591. eCollection 2014. PMID: 25057443; PMCID: PMC4092313.
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Mob Genet Elements. 2014 Jun 23;4:e29591. doi: 10.4161/mge.29591. eCollection 2014.

Driving DNA transposition by lentiviral protein transduction.

Mobile genetic elements

Yujia Cai, Jacob Giehm Mikkelsen

Affiliations

  1. Department of Biomedicine; Aarhus University; Aarhus C, Denmark.

PMID: 25057443 PMCID: PMC4092313 DOI: 10.4161/mge.29591

Abstract

Gene vectors derived from DNA transposable elements have become powerful molecular tools in biomedical research and are slowly moving into the clinic as carriers of therapeutic genes. Conventional uses of DNA transposon-based gene vehicles rely on the intracellular production of the transposase protein from transfected nucleic acids. The transposase mediates mobilization of the DNA transposon, which is typically provided in the context of plasmid DNA. In recent work, we established lentiviral protein transduction from Gag precursors as a new strategy for direct delivery of the transposase protein. Inspired by the natural properties of infecting viruses to carry their own enzymes, we loaded lentivirus-derived particles not only with vector genomes carrying the DNA transposon vector but also with hundreds of transposase subunits. Such particles were found to drive efficient transposition of the

Keywords: DNA transposition; IDLV; Sleeping Beauty; lentiviral vector; piggyBac; protein transduction

References

  1. BMC Biotechnol. 2006 Dec 27;6:52 - PubMed
  2. Nat Methods. 2012 Jul 01;9(8):805-7 - PubMed
  3. Mol Ther. 2009 Jul;17(7):1205-14 - PubMed
  4. Cell. 2005 Aug 12;122(3):473-83 - PubMed
  5. J Virol. 2004 Oct;78(19):10803-13 - PubMed
  6. J Cell Biol. 2003 Sep 29;162(7):1233-44 - PubMed
  7. Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9233-7 - PubMed
  8. Mol Ther. 2009 Oct;17(10):1743-53 - PubMed
  9. Mol Ther. 2003 Oct;8(4):654-65 - PubMed
  10. Mol Ther. 2007 Jan;15(1):139-45 - PubMed
  11. Nucleic Acids Res. 2007;35(7):e50 - PubMed
  12. BMC Dermatol. 2011 Feb 27;11:5 - PubMed
  13. Mol Ther. 2007 Jul;15(7):1280-7 - PubMed
  14. Mol Ther. 2011 Sep;19(9):1636-44 - PubMed
  15. Nucleic Acids Res. 2014 Feb;42(4):e28 - PubMed
  16. Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7520-5 - PubMed
  17. Virology. 2006 Feb 5;345(1):115-26 - PubMed
  18. Elife. 2014 Apr 24;3:e01911 - PubMed
  19. J Virol. 2006 Feb;80(4):1939-48 - PubMed
  20. J Virol. 1997 Jan;71(1):458-64 - PubMed
  21. PLoS One. 2012;7(5):e36658 - PubMed
  22. Nat Genet. 2000 May;25(1):35-41 - PubMed
  23. Mol Ther. 2009 Jul;17(7):1136-44 - PubMed
  24. J Virol. 1996 Jan;70(1):37-46 - PubMed
  25. PLoS One. 2011;6(11):e27234 - PubMed
  26. BMC Biotechnol. 2011 Apr 07;11:33 - PubMed
  27. PLoS One. 2012;7(12):e52473 - PubMed
  28. Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15453-8 - PubMed
  29. Cell. 2008 May 16;133(4):742, 742.e1 - PubMed
  30. Mol Ther. 2010 Oct;18(10):1814-21 - PubMed
  31. DNA Cell Biol. 2000 Mar;19(3):179-88 - PubMed
  32. J Virol. 1983 Aug;47(2):287-300 - PubMed
  33. Nature. 2005 Jul 14;436(7048):221-6 - PubMed
  34. Biomaterials. 2011 Sep;32(26):6264-76 - PubMed
  35. J Mol Biol. 2002 May 17;318(5):1221-35 - PubMed
  36. PLoS One. 2013 Oct 08;8(10):e76771 - PubMed
  37. PLoS One. 2008 Apr 16;3(4):e1995 - PubMed
  38. Mol Ther. 2005 Dec;12(6):1185-96 - PubMed
  39. Mol Ther Nucleic Acids. 2013 Feb 26;2:e74 - PubMed
  40. Cell. 1997 Nov 14;91(4):501-10 - PubMed
  41. J Virol. 1990 May;64(5):2265-79 - PubMed
  42. Gene Ther. 2011 Sep;18(9):936-41 - PubMed
  43. Transgenic Res. 2011 Jun;20(3):533-45 - PubMed
  44. EMBO J. 1997 Aug 15;16(16):5123-38 - PubMed
  45. Nucleic Acids Res. 2013 Mar 1;41(5):e61 - PubMed
  46. Nucleic Acids Res. 2006 Jan 30;34(2):e16 - PubMed
  47. Hum Gene Ther. 2010 May;21(5):589-602 - PubMed
  48. Mol Ther. 2011 Aug;19(8):1499-510 - PubMed
  49. Nucleic Acids Res. 2011 Sep 1;39(16):7147-60 - PubMed
  50. J Virol. 1995 Jun;69(6):3389-98 - PubMed
  51. Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4062-7 - PubMed
  52. Virology. 1996 May 1;219(1):307-13 - PubMed
  53. Nature. 2005 Jul 14;436(7048):272-6 - PubMed
  54. Nucleic Acids Res. 2011 Sep 1;39(17):7610-9 - PubMed
  55. J Biol Chem. 1998 Apr 3;273(14):8009-16 - PubMed
  56. Virology. 1999 Mar 1;255(1):77-85 - PubMed
  57. J Virol. 1990 Sep;64(9):4169-79 - PubMed
  58. Nature. 2008 Jul 10;454(7201):236-40 - PubMed
  59. Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):7805-10 - PubMed
  60. Cancer Res. 2008 Apr 15;68(8):2961-71 - PubMed
  61. J Biomed Sci. 2013 Dec 09;20:92 - PubMed
  62. EMBO J. 1997 Aug 15;16(16):5113-22 - PubMed
  63. FEBS Lett. 2013 Jun 19;587(12):1693-702 - PubMed
  64. J Immunother. 2013 Feb;36(2):112-23 - PubMed
  65. Virology. 2003 Aug 15;313(1):91-104 - PubMed
  66. Nat Med. 2006 Mar;12(3):348-53 - PubMed
  67. J Cell Biol. 2002 Nov 11;159(3):441-52 - PubMed
  68. Hum Gene Ther. 2000 Mar 1;11(4):537-46 - PubMed
  69. Nat Genet. 2009 Jun;41(6):753-61 - PubMed
  70. Cancer Res. 2006 Jan 15;66(2):627-31 - PubMed
  71. J Virol. 2007 Dec;81(23):12899-910 - PubMed
  72. Sci Rep. 2012;2:359 - PubMed
  73. J Virol. 2004 Feb;78(3):1301-13 - PubMed
  74. Sci Transl Med. 2013 Jan 2;5(166):166ra1 - PubMed
  75. Mol Cell Biol. 2004 Oct;24(20):9239-47 - PubMed
  76. Nat Biotechnol. 2002 Oct;20(10):999-1005 - PubMed

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