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Iketaki Y, Kitagawa K, Hidaka K, et al. Fabrication of optical multilayer for two-color phase plate in super-resolution microscope. Rev Sci Instrum. 2014;85(7):073701doi: 10.1063/1.4885465.
Iketaki, Y., Kitagawa, K., Hidaka, K., Kato, N., Hirabayashi, A., & Bokor, N. (2014). Fabrication of optical multilayer for two-color phase plate in super-resolution microscope. The Review of scientific instruments, 85(7), 073701. https://doi.org/10.1063/1.4885465
Iketaki, Yoshinori, et al. "Fabrication of optical multilayer for two-color phase plate in super-resolution microscope." The Review of scientific instruments vol. 85,7 (2014): 073701. doi: https://doi.org/10.1063/1.4885465
Iketaki Y, Kitagawa K, Hidaka K, Kato N, Hirabayashi A, Bokor N. Fabrication of optical multilayer for two-color phase plate in super-resolution microscope. Rev Sci Instrum. 2014 Jul;85(7):073701. doi: 10.1063/1.4885465. PMID: 25085140.
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Rev Sci Instrum. 2014 Jul;85(7):073701. doi: 10.1063/1.4885465.

Fabrication of optical multilayer for two-color phase plate in super-resolution microscope.

The Review of scientific instruments

Yoshinori Iketaki, Katsuichi Kitagawa, Kohjiro Hidaka, Naoki Kato, Akira Hirabayashi, Nandor Bokor

Affiliations

  1. Olympus Corporation, 2-3 Kuboyama-cho, Hachioji 192-8512, Japan.
  2. Toray Engineering Co. Ltd., 1-1-45 Oe, Otsu 520-2141, Japan.
  3. Ceratech Japan Co., Ltd., 500 Okada, Shinonoi, Nagano 381-2295, Japan.
  4. Department of Physics, Budapest University of Technology and Economics, Budapest 1111, Hungary.

PMID: 25085140 DOI: 10.1063/1.4885465

Abstract

In super-resolution microscopy based on fluorescence depletion, the two-color phase plate (TPP) is an indispensable optical element, which can independently control the phase shifts for two beams of different color, i.e., the pump and erase beams. By controlling a phase shift of the erase beam through the TPP, the erase beam can be modulated into a doughnut shape, while the pump beam maintains the initial Gaussian shape. To obtain a reliable optical multiplayer (ML) for the TPP, we designed a ML with only two optical layers by performing numerical optimization. The measured phase shifts generated by the fabricated ML using interferometry correspond to the design values. The beam profiles in the focal plane are also consistent with theoretical results. Although the fabricated ML consists of only two optical layers, the ML can provide a suitable phase modulation function for the TPP in a practical super-resolution microscope.

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