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Herweijer G, Kyloh M, Beckett EA, et al. Characterization of primary afferent spinal innervation of mouse uterus. Front Neurosci. 2014;8:202doi: 10.3389/fnins.2014.00202.
Herweijer, G., Kyloh, M., Beckett, E. A., Dodds, K. N., & Spencer, N. J. (2014). Characterization of primary afferent spinal innervation of mouse uterus. Frontiers in neuroscience, 8202. https://doi.org/10.3389/fnins.2014.00202
Herweijer, Geraldine, et al. "Characterization of primary afferent spinal innervation of mouse uterus." Frontiers in neuroscience vol. 8 (2014): 202. doi: https://doi.org/10.3389/fnins.2014.00202
Herweijer G, Kyloh M, Beckett EA, Dodds KN, Spencer NJ. Characterization of primary afferent spinal innervation of mouse uterus. Front Neurosci. 2014 Jul 28;8:202. doi: 10.3389/fnins.2014.00202. eCollection 2014. PMID: 25120416; PMCID: PMC4112786.
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Front Neurosci. 2014 Jul 28;8:202. doi: 10.3389/fnins.2014.00202. eCollection 2014.

Characterization of primary afferent spinal innervation of mouse uterus.

Frontiers in neuroscience

Geraldine Herweijer, Melinda Kyloh, Elizabeth A H Beckett, Kelsi N Dodds, Nick J Spencer

Affiliations

  1. Discipline of Human Physiology and Centre for Neuroscience, Flinders University Adelaide, SA, Australia.
  2. Discipline of Physiology, School of Medical Sciences, University of Adelaide Adelaide, SA, Australia.

PMID: 25120416 PMCID: PMC4112786 DOI: 10.3389/fnins.2014.00202

Abstract

The primary afferent innervation of the uterus is incompletely understood. The aim of this study was to identify the location and characteristics of primary afferent neurons that innervate the uterine horn of mice and correlate the different morphological types of putative primary afferent nerve endings, immunoreactive to the sensory marker, calcitonin gene related peptide (CGRP). Using retrograde tracing, injection of 5-10 μL of 1,1'-didodecyl-3,3,3,3'-tetramethylindocarbocyanine perchlorate (DiI) into discrete single sites in each uterine horn revealed a biomodal distribution of sensory neurons in dorsal root ganglia (DRG) with peak labeling occurring between T13-L3 and a second smaller peak between L6-S1. The mean cross sectional area of labeled cells was 463 μm(2) ± s.e.m. A significantly greater proportion of labeled neurons consisted of small cell bodies (<300 μm(2)) in the sacral spinal cord (S2) compared with peak labeling at the lumbar (L2) region. In both sections and whole mount preparations, immunohistochemical staining for CGRP revealed substantial innervation of the uterus by CGRP-positive nerve fibers located primarily at the border between the circular and longitudinal muscle layers (N = 4). The nerve endings were classified into three distinct types: "single," "branching," or "complex," that often aligned preferentially in either the circular or longitudinal axis of the smooth muscles. Complex endings were often associated with mesenteric vessels. We have identified that the cell bodies of primary afferent neurons innervating the mouse uterus lie primarily in DRG at L2 and S1 spinal levels. Also, the greatest density of CGRP immunoreactivity lies within the myometrium, with at least three different morphological types of nerve endings identified. These findings will facilitate further investigations into the mechanisms underlying sensory transduction in mouse uterus.

Keywords: CGRP; dorsal root ganglion; pain; spinal afferent; uterus

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