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Needhamsen M, White RB, Giles KM, et al. Regulation of Human PAX6 Expression by miR-7. Evol Bioinform Online. 2014;10:107-13doi: 10.4137/EBO.S13739.
Needhamsen, M., White, R. B., Giles, K. M., Dunlop, S. A., & Thomas, M. G. (2014). Regulation of Human PAX6 Expression by miR-7. Evolutionary bioinformatics online, 10107-13. https://doi.org/10.4137/EBO.S13739
Needhamsen, Maria, et al. "Regulation of Human PAX6 Expression by miR-7." Evolutionary bioinformatics online vol. 10 (2014): 107-13. doi: https://doi.org/10.4137/EBO.S13739
Needhamsen M, White RB, Giles KM, Dunlop SA, Thomas MG. Regulation of Human PAX6 Expression by miR-7. Evol Bioinform Online. 2014 Jul 14;10:107-13. doi: 10.4137/EBO.S13739. eCollection 2014. PMID: 25089088; PMCID: PMC4116382.
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Evol Bioinform Online. 2014 Jul 14;10:107-13. doi: 10.4137/EBO.S13739. eCollection 2014.

Regulation of Human PAX6 Expression by miR-7.

Evolutionary bioinformatics online

Maria Needhamsen, Robert B White, Keith M Giles, Sarah A Dunlop, Meghan G Thomas

Affiliations

  1. Parkinson's Centre (ParkC), School of Medical Sciences, Edith Cowan University, Joondalup, Western Australia, Australia. ; Experimental and Regenerative Neurosciences (EaRN), School of Animal Biology, University of Western Australia, Crawley, Western Australia, Australia.
  2. Parkinson's Centre (ParkC), School of Medical Sciences, Edith Cowan University, Joondalup, Western Australia, Australia. ; Experimental and Regenerative Neurosciences (EaRN), School of Animal Biology, University of Western Australia, Crawley, Western Australia, Australia. ; School of Anatomy, Physiology and Human Biology, University of Western Australia, Crawley, Western Australia, Australia.
  3. Harry Perkins Institute of Medical Research, Nedlands, Western Australia, Australia.
  4. Experimental and Regenerative Neurosciences (EaRN), School of Animal Biology, University of Western Australia, Crawley, Western Australia, Australia.

PMID: 25089088 PMCID: PMC4116382 DOI: 10.4137/EBO.S13739

Abstract

The paired box gene 6 (PAX6) is a powerful mediator of eye and brain organogenesis whose spatiotemporal expression is exquisitely controlled by multiple mechanisms, including post-transcriptional regulation by microRNAs (miRNAs). In the present study, we use bioinformatic predictions to identify three candidate microRNA-7 (miR-7) target sites in the human PAX6 3' untranslated region (3'-UTR) and demonstrate that two of them are functionally active in a human cell line. Furthermore, transient transfection of cells with synthetic miR-7 inhibits PAX6 protein expression but does not alter levels of PAX6 mRNA, suggesting that miR-7 induces translational repression of PAX6. Finally, a comparison of PAX6 3'-UTRs across species reveals that one of the functional miR-7 target sites is conserved, whereas the second functional target site is found only in primates. Thus, the interaction between PAX6 and miR-7 appears to be highly conserved; however, the precise number of sites through which this interaction occurs may have expanded throughout evolution.

Keywords: 3′-UTR; PAX6; miR-7; miRNA

References

  1. Development. 2009 Dec;136(23):4055-63 - PubMed
  2. Cancer Res. 2013 Sep 15;73(18):5609-12 - PubMed
  3. Curr Opin Neurobiol. 2010 Aug;20(4):457-65 - PubMed
  4. Stem Cells Dev. 2013 May 15;22(10):1522-40 - PubMed
  5. BMB Rep. 2011 Sep;44(9):595-600 - PubMed
  6. Neuron. 1998 Nov;21(5):1031-44 - PubMed
  7. PLoS Biol. 2013;11(5):e1001554 - PubMed
  8. PLoS One. 2014 Jan 15;9(1):e85738 - PubMed
  9. Mol Cell. 2007 Jul 6;27(1):91-105 - PubMed
  10. Biochem J. 2012 Jan 1;441(1):367-77 - PubMed
  11. Nucleic Acids Res. 2014 Jan;42(1):458-74 - PubMed
  12. Oncogene. 2008 Oct 9;27(46):6068-74 - PubMed
  13. J Neurosci. 1994 Mar;14(3 Pt 2):1395-412 - PubMed
  14. Nat Genet. 2007 Oct;39(10):1278-84 - PubMed
  15. Cell Stem Cell. 2010 Jul 2;7(1):90-100 - PubMed
  16. J Biol Chem. 2009 Feb 27;284(9):5731-41 - PubMed
  17. Development. 2012 Aug;139(16):3021-31 - PubMed
  18. J Biol Chem. 2009 Oct 2;284(40):27524-32 - PubMed
  19. Biochem J. 2010 Nov 15;432(1):199-205 - PubMed
  20. J Biol Chem. 2003 Jan 31;278(5):2937-46 - PubMed
  21. Cell. 1997 Jul 11;90(1):169-80 - PubMed
  22. Nat Neurosci. 2012 Jun 24;15(8):1120-6 - PubMed
  23. Cell. 2009 Jan 23;136(2):215-33 - PubMed
  24. Invest Ophthalmol Vis Sci. 2007 Sep;48(9):3962-7 - PubMed
  25. Cell. 2005 Jan 14;120(1):15-20 - PubMed
  26. Prostate. 2010 Feb 1;70(2):190-9 - PubMed
  27. Immunol Cell Biol. 2008 Mar-Apr;86(3):271-6 - PubMed
  28. PLoS Biol. 2005 Mar;3(3):e85 - PubMed
  29. Cell. 2003 Dec 26;115(7):787-98 - PubMed
  30. Methods. 2001 Dec;25(4):402-8 - PubMed
  31. Diabetes. 2013 Mar;62(3):887-95 - PubMed
  32. Cell. 2004 Jan 23;116(2):281-97 - PubMed
  33. Trends Biochem Sci. 2003 Feb;28(2):91-8 - PubMed

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