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Crowell KL, Baker ES, Payne SH, et al. Increasing Confidence of LC-MS Identifications by Utilizing Ion Mobility Spectrometry. Int J Mass Spectrom. 2013;354:312-317doi: 10.1016/j.ijms.2013.06.028.
Crowell, K. L., Baker, E. S., Payne, S. H., Ibrahim, Y. M., Monroe, M. E., Slysz, G. W., LaMarche, B. L., Petyuk, V. A., Piehowski, P. D., Danielson, W. F., Anderson, G. A., & Smith, R. D. (2013). Increasing Confidence of LC-MS Identifications by Utilizing Ion Mobility Spectrometry. International journal of mass spectrometry, 354312-317. https://doi.org/10.1016/j.ijms.2013.06.028
Crowell, Kevin L, et al. "Increasing Confidence of LC-MS Identifications by Utilizing Ion Mobility Spectrometry." International journal of mass spectrometry vol. 354 (2013): 312-317. doi: https://doi.org/10.1016/j.ijms.2013.06.028
Crowell KL, Baker ES, Payne SH, Ibrahim YM, Monroe ME, Slysz GW, LaMarche BL, Petyuk VA, Piehowski PD, Danielson WF, Anderson GA, Smith RD. Increasing Confidence of LC-MS Identifications by Utilizing Ion Mobility Spectrometry. Int J Mass Spectrom. 2013 Nov 15;354:312-317. doi: 10.1016/j.ijms.2013.06.028. PMID: 25089116; PMCID: PMC4114398.
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Int J Mass Spectrom. 2013 Nov 15;354:312-317. doi: 10.1016/j.ijms.2013.06.028.

Increasing Confidence of LC-MS Identifications by Utilizing Ion Mobility Spectrometry.

International journal of mass spectrometry

Kevin L Crowell, Erin S Baker, Samuel H Payne, Yehia M Ibrahim, Matthew E Monroe, Gordon W Slysz, Brian L LaMarche, Vladislav A Petyuk, Paul D Piehowski, William F Danielson, Gordon A Anderson, Richard D Smith

Affiliations

  1. Pacific Northwest National Laboratory, Richland, WA 99352.

PMID: 25089116 PMCID: PMC4114398 DOI: 10.1016/j.ijms.2013.06.028

Abstract

Ion mobility spectrometry in conjunction with liquid chromatography separations and mass spectrometry offers a range of new possibilities for analyzing complex biological samples. To fully utilize the information obtained from these three measurement dimensions, informatics tools based on the accurate mass and time tag methodology were modified to incorporate ion mobility spectrometry drift times for peptides observed in human serum. In this work a reference human serum database was created for 12,139 peptides and populated with the monoisotopic mass, liquid chromatography normalized elution time, and ion mobility spectrometry drift time(s) for each. We demonstrate that the use of three dimensions for peak matching during the peptide identification process resulted in an increased numbers of identifications and a lower false discovery rate relative to only using the mass and normalized elution time dimensions.

Keywords: AMT tag; LC-IMS-MS; LC-MS; drift time; false discovery rate; ion mobility spectrometry; mass spectrometry; peptide identification

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