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Li D, Fan J, Li Z, et al. DNA methylation dynamics in the rat EGF gene promoter after partial hepatectomy. Genet Mol Biol. 2014;37(2):439-43doi: 10.1590/s1415-47572014000300017.
Li, D., Fan, J., Li, Z., & Xu, C. (2014). DNA methylation dynamics in the rat EGF gene promoter after partial hepatectomy. Genetics and molecular biology, 37(2), 439-43. https://doi.org/10.1590/s1415-47572014000300017
Li, Deming, et al. "DNA methylation dynamics in the rat EGF gene promoter after partial hepatectomy." Genetics and molecular biology vol. 37,2 (2014): 439-43. doi: https://doi.org/10.1590/s1415-47572014000300017
Li D, Fan J, Li Z, Xu C. DNA methylation dynamics in the rat EGF gene promoter after partial hepatectomy. Genet Mol Biol. 2014 Jun;37(2):439-43. doi: 10.1590/s1415-47572014000300017. PMID: 25071410; PMCID: PMC4094617.
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Genet Mol Biol. 2014 Jun;37(2):439-43. doi: 10.1590/s1415-47572014000300017.

DNA methylation dynamics in the rat EGF gene promoter after partial hepatectomy.

Genetics and molecular biology

Deming Li, Jinyu Fan, Ziwei Li, Cunshuan Xu

Affiliations

  1. Key Laboratory for Cell Differentiation Regulation , Xinxiang , China . ; College of Life Science , Henan Normal University , Xinxiang , China .

PMID: 25071410 PMCID: PMC4094617 DOI: 10.1590/s1415-47572014000300017

Abstract

Epidermal growth factor (EGF), a multifunctional growth factor, is a regulator in a wide variety of physiological processes. EGF plays an important role in the regulation of liver regeneration. This study was aimed at investigating the methylation level of EGF gene throughout liver regeneration. DNA of liver tissue from control rats and partial hepatectomy (PH) rats at 10 time points was extracted and a 354 bp fragment including 10 CpG sites from the transcription start was amplified after DNA was modified by sodium bisulfate. The result of sequencing suggested that methylation ratio of four CpG sites was found to be significantly changed when PH group was compared to control group, in particular two of them were extremely striking. mRNA expression of EGF was down-regulated in total during liver regeneration. We think that the rat EGF promoter region is regulated by variation in DNA methylation during liver regeneration.

Keywords: epidermal growth factor; liver regeneration; methylation dynamics

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