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Liu W, Fan J, Li J, et al. SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida. Front Genet. 2014;5:228doi: 10.3389/fgene.2014.00228.
Liu, W., Fan, J., Li, J., Song, Y., Li, Q., Zhang, Y., & Xue, Y. (2014). SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida. Frontiers in genetics, 5228. https://doi.org/10.3389/fgene.2014.00228
Liu, Wei, et al. "SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida." Frontiers in genetics vol. 5 (2014): 228. doi: https://doi.org/10.3389/fgene.2014.00228
Liu W, Fan J, Li J, Song Y, Li Q, Zhang Y, Xue Y. SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida. Front Genet. 2014 Jul 22;5:228. doi: 10.3389/fgene.2014.00228. eCollection 2014. PMID: 25101113; PMCID: PMC4106197.
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Front Genet. 2014 Jul 22;5:228. doi: 10.3389/fgene.2014.00228. eCollection 2014.

SCF(SLF)-mediated cytosolic degradation of S-RNase is required for cross-pollen compatibility in S-RNase-based self-incompatibility in Petunia hybrida.

Frontiers in genetics

Wei Liu, Jiangbo Fan, Junhui Li, Yanzhai Song, Qun Li, Yu'e Zhang, Yongbiao Xue

Affiliations

  1. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research Beijing, China ; University of Chinese Academy of Sciences Beijing, China.
  2. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research Beijing, China.

PMID: 25101113 PMCID: PMC4106197 DOI: 10.3389/fgene.2014.00228

Abstract

Many flowering plants adopt self-incompatibility (SI) to maintain their genetic diversity. In species of Solanaceae, Plantaginaceae, and Rosaceae, SI is genetically controlled by a single S-locus with multiple haplotypes. The S-locus has been shown to encode S-RNases expressed in pistil and multiple SLF (S-locus F-box) proteins in pollen controlling the female and male specificity of SI, respectively. S-RNases appear to function as a cytotoxin to reject self-pollen. In addition, SLFs have been shown to form SCF (SKP1/Cullin1/F-box) complexes to serve as putative E3 ubiquitin ligase to interact with S-RNases. Previously, two different mechanisms, the S-RNase degradation and the S-RNase compartmentalization, have been proposed as the restriction mechanisms of S-RNase cytotoxicity allowing compatible pollination. In this study, we have provided several lines of evidence in support of the S-RNase degradation mechanism by a combination of cellular, biochemical and molecular biology approaches. First, both immunogold labeling and subcellular fractionation assays showed that two key pollen SI factors, PhS3L-SLF1 and PhSSK1 (SLF-interacting SKP1-like1) from Petunia hybrida, a Solanaceous species, are co-localized in cytosols of both pollen grains and tubes. Second, PhS3L-RNases are mainly detected in the cytosols of both self and non-self-pollen tubes after pollination. Third, we found that PhS-RNases selectively interact with PhSLFs by yeast two-hybrid and co-immunoprecipitation assays. Fourth, S-RNases are specifically degraded in compatible pollen tubes by non-self SLF action. Taken together, our results demonstrate that SCF(SLF-mediated) non-self S-RNase degradation occurs in the cytosol of pollen tube through the ubiquitin/26S proteasome system serving as the major mechanism to neutralize S-RNase cytotoxicity during compatible pollination in P. hybrida.

Keywords: S-RNase localization; SCFSLF; cross-pollen compatibility; self-incompatibility; self-pollen incompatibility; ubiquitin/26S proteasome system

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