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Chaijaroenkul W, Thiengsusuk A, Rungsihirunrat K, et al. Proteomics analysis of antimalarial targets of Garcinia mangostana Linn. Asian Pac J Trop Biomed. 2014;4(7):515-9doi: 10.12980/APJTB.4.2014APJTB-2014-0043.
Chaijaroenkul, W., Thiengsusuk, A., Rungsihirunrat, K., Ward, S. A., & Na-Bangchang, K. (2014). Proteomics analysis of antimalarial targets of Garcinia mangostana Linn. Asian Pacific journal of tropical biomedicine, 4(7), 515-9. https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0043
Chaijaroenkul, Wanna, et al. "Proteomics analysis of antimalarial targets of Garcinia mangostana Linn." Asian Pacific journal of tropical biomedicine vol. 4,7 (2014): 515-9. doi: https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0043
Chaijaroenkul W, Thiengsusuk A, Rungsihirunrat K, Ward SA, Na-Bangchang K. Proteomics analysis of antimalarial targets of Garcinia mangostana Linn. Asian Pac J Trop Biomed. 2014 Jul;4(7):515-9. doi: 10.12980/APJTB.4.2014APJTB-2014-0043. PMID: 25183269; PMCID: PMC4032823.
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Asian Pac J Trop Biomed. 2014 Jul;4(7):515-9. doi: 10.12980/APJTB.4.2014APJTB-2014-0043.

Proteomics analysis of antimalarial targets of Garcinia mangostana Linn.

Asian Pacific journal of tropical biomedicine

Wanna Chaijaroenkul, Artitiya Thiengsusuk, Kanchana Rungsihirunrat, Stephen Andrew Ward, Kesara Na-Bangchang

Affiliations

  1. Chulabhorn International College of Medicine, Thammasat University (Rangsit Campus), Pathumtani 12121, Thailand.
  2. College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
  3. Molecular and Biochemical Parasitology, Liverpool School of Tropical Medicine, University of Liverpool, Liverpool L3 5QA, United Kingdom.

PMID: 25183269 PMCID: PMC4032823 DOI: 10.12980/APJTB.4.2014APJTB-2014-0043

Abstract

OBJECTIVE: To investigate possible protein targets for antimalarial activity of Garcinia mangostana Linn. (G. mangostana) (pericarp) in 3D7 Plasmodium falciparum clone using 2-dimensional electrophoresis and liquid chromatography mass-spectrometry (LC/MS/MS).

METHODS: 3D7 Plasmodium falciparum was exposed to the crude ethanolic extract of G. mangostana Linn. (pericarp) at the concentrations of 12µg/mL (IC50 level: concentration that inhibits parasite growth by 50%) and 30 µg/mL (IC90 level: concentration that inhibits parasite growth by 90%) for 12 h. Parasite proteins were separated by 2-dimensional electrophoresis and identified by LC/MS/MS.

RESULTS: At the IC50 concentration, about 82% of the expressed parasite proteins were matched with the control (non-exposed), while at the IC90 concentration, only 15% matched proteins were found. The selected protein spots from parasite exposed to the plant extract at the concentration of 12 µg/mL were identified as enzymes that play role in glycolysis pathway, i.e., phosphoglycerate mutase putative, L-lactate dehydrogenase/glyceraldehyde-3-phosphate dehydrogenase, and fructose-bisphosphate aldolase/phosphoglycerate kinase. The proteosome was found in parasite exposed to 30 µg/mL of the extract.

CONCLUSIONS: Results suggest that proteins involved in the glycolysis pathway may be the targets for antimalarial activity of G. mangostana Linn. (pericarp).

Keywords: Garcinia mangostana Linn.; Malaria; Proteomics

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