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Haghighi F, Shahcheraghi F, Abbasi E, et al. Genetic Profile Variation in Vaccine Strains and Clinical Isolates of Bordetella pertussis Recovered from Iranian Patients. Avicenna J Med Biotechnol. 2014;6(3):178-84
Haghighi, F., Shahcheraghi, F., Abbasi, E., Eshraghi, S. S., Zeraati, H., Mousavi, S. A., Asgarian-Omran, H., Douraghi, M., & Shokri, F. (2014). Genetic Profile Variation in Vaccine Strains and Clinical Isolates of Bordetella pertussis Recovered from Iranian Patients. Avicenna journal of medical biotechnology, 6(3), 178-84.
Haghighi, Faezeh, et al. "Genetic Profile Variation in Vaccine Strains and Clinical Isolates of Bordetella pertussis Recovered from Iranian Patients." Avicenna journal of medical biotechnology vol. 6,3 (2014): 178-84.
Haghighi F, Shahcheraghi F, Abbasi E, Eshraghi SS, Zeraati H, Mousavi SA, Asgarian-Omran H, Douraghi M, Shokri F. Genetic Profile Variation in Vaccine Strains and Clinical Isolates of Bordetella pertussis Recovered from Iranian Patients. Avicenna J Med Biotechnol. 2014 Jul;6(3):178-84. PMID: 25215182; PMCID: PMC4147105.
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Avicenna J Med Biotechnol. 2014 Jul;6(3):178-84.

Genetic Profile Variation in Vaccine Strains and Clinical Isolates of Bordetella pertussis Recovered from Iranian Patients.

Avicenna journal of medical biotechnology

Faezeh Haghighi, Fereshteh Shahcheraghi, Ebrahim Abbasi, Seyed Saeed Eshraghi, Hojjat Zeraati, Seyed Ali Javad Mousavi, Hossein Asgarian-Omran, Masoumeh Douraghi, Fazel Shokri

Affiliations

  1. Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  2. Department of Bacteriology and Microbiology, Pasteur Institute of Iran, Tehran, Iran.
  3. Department of Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran.
  4. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
  5. Department of Pulmonology, Hazrat Rasool Hospital, Iran University of Medical Sciences, Tehran, Iran.
  6. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran ; Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
  7. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran ; Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

PMID: 25215182 PMCID: PMC4147105

Abstract

BACKGROUND: Re-emergence of pertussis has been reported in Iran despite a high rate of vaccination coverage. Low efficacy of the vaccine might be due to the genetic divergence between clinical versus vaccine strains. In the current study, the genetic profiles of clinical isolates and vaccine strains of Bordetella pertussis (B. pertussis) were assessed by using Pulsed Field Gel Electrophoresis (PFGE).

METHODS: Following phenotypic and molecular identification of isolates, XbaI-digested genomic DNA of 5 clinical isolates, 2 vaccine strains and a Tohama I strain were analyzed by PFGE along with B. parapertussis as a control.

RESULTS: Seven distinct PFGE profiles were found among all examined isolates/strains. In 5 clinical isolates, 4 profiles were identified whereas the vaccine strains displayed 2 distinct profiles. The reference strain, Tohama I had a distinct profile. Vaccine and clinical profiles had low similarity, with relatedness of approximately 40%.

CONCLUSION: The genetic profiles of B. pertussis were different between circulating isolates and vaccine strains used in the national vaccination programs. Since new genetic profiles of B. pertussis can be disseminated periodically, the profiles of isolates circulating in the population should be monitored over the course of the re-emergence.

Keywords: Bordetella pertussis; PFGE profile; Vaccination; Whooping cough

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