World J Microbiol Biotechnol. 2011 Jun;27(6):1313-21. doi: 10.1007/s11274-010-0579-0. Epub 2010 Oct 16.
Evaluation of bacteria isolated from rice rhizosphere for biological control of charcoal rot of sorghum caused by Macrophomina phaseolina (Tassi) Goid.
World journal of microbiology & biotechnology
Subramaniam Gopalakrishnan, Pagidi Humayun, Bandru Keerthi Kiran, Iyer Girish Kumar Kannan, Meesala Sree Vidya, Kanala Deepthi, Om Rupela
Affiliations
Affiliations
- Global Theme-Crop Improvement, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502 324, Andhra Pradesh, India, [email protected].
PMID: 25187130
DOI: 10.1007/s11274-010-0579-0
Abstract
A total of 360 bacteria, isolated from the rhizospheres of a system of rice intensification (SRI) fields, were characterized for the production of siderophore, fluorescence, indole acetic acid (IAA), hydrocyanic acid (HCN) and solubilization of phosphorus. Of them, seven most promising isolates (SRI-156, -158, -178, -211, -229, -305 and -360) were screened for their antagonistic potential against Macrophomina phaseolina (causes charcoal rot in sorghum) by dual culture assay, blotter paper assay and in greenhouse. All the seven isolates inhibited M. phaseolina in dual culture assay, whereas six isolates solubilized phosphorous (except SRI-360), all seven produced siderophore, four produced fluorescence (except SRI-178, -229 and -305), six produced IAA (except SRI-305) and five produced HCN (except SRI-158 and -305). In the blotter paper assay, no charcoal rot infection was observed in SRI-156-treated sorghum roots, indicating complete inhibition of the pathogen, while the roots treated with the other isolates showed 49-76% lesser charcoal rot infection compared to the control. In the antifungal activity test (in green house on sorghum), all the isolates increased shoot dry mass by 15-23% and root dry mass by 15-20% (except SRI-158 and -360), over the control. In order to confirm the plant growth-promoting (PGP) traits of the isolates, the green house experiment was repeated but, in the absence of M. phaseolina. The results further confirmed the PGP traits of the isolates as evidenced by increases in shoot and root dry mass, 22-100% and 5-20%, respectively, over the control. The sequences of 16S rDNA gene of the isolates SRI-156, -158, -178, -211, -229, -305 and -360 were matched with Pseudomonas plecoglossicida, Brevibacterium antiquum, Bacillus altitudinis, Enterobacter ludwigii, E. ludwigii, Acinetobacter tandoii and P. monteilii, respectively in BLAST analysis. This study indicates that the selected bacterial isolates have the potential for PGP and control of charcoal rot disease in sorghum.
References
- Annu Rev Phytopathol. 2002;40:309-48 - PubMed
- Annu Rev Phytopathol. 1993;31:53-80 - PubMed
- J Lab Clin Med. 1954 Aug;44(2):301-7 - PubMed
- Mol Biol Evol. 1987 Jul;4(4):406-25 - PubMed
- Appl Environ Microbiol. 1991 Nov;57(11):3270-7 - PubMed
- J Appl Microbiol. 2004;96(3):473-80 - PubMed
- Biotechnol Adv. 1999 Oct;17(4-5):319-39 - PubMed
- Phytopathology. 2002 Dec;92(12):1337-43 - PubMed
- Microbiol Res. 2001;156(1):87-93 - PubMed
- Syst Appl Microbiol. 2005 Jan;28(1):66-76 - PubMed
- Appl Environ Microbiol. 2002 Aug;68(8):3795-801 - PubMed
- Microbiol Res. 2001;156(3):209-23 - PubMed
- FEMS Microbiol Lett. 2005 Jan 15;242(2):249-56 - PubMed
- Mol Plant Microbe Interact. 1992 Jul-Aug;5(4):330-9 - PubMed
- Can J Microbiol. 1996 Mar;42(3):207-20 - PubMed
- Curr Microbiol. 2006 Apr;52(4):310-6 - PubMed
- Microbiol Res. 2008;163(2):234-42 - PubMed
- Anal Biochem. 1987 Jan;160(1):47-56 - PubMed
- Lett Appl Microbiol. 2005;40(4):260-8 - PubMed
- Nucleic Acids Res. 1997 Dec 15;25(24):4876-82 - PubMed
- J Mol Biol. 1990 Oct 5;215(3):403-10 - PubMed
- Can J Microbiol. 2001 Oct;47(10):916-24 - PubMed
- Environ Microbiol. 2006 Jan;8(1):1-10 - PubMed
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