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Warnock JJ, Bobe G, Duesterdieck-Zellmer KF. Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs. PeerJ. 2014;2:e581doi: 10.7717/peerj.581.
Warnock, J. J., Bobe, G., & Duesterdieck-Zellmer, K. F. (2014). Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs. PeerJ, 2e581. https://doi.org/10.7717/peerj.581
Warnock, Jennifer J, et al. "Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs." PeerJ vol. 2 (2014): e581. doi: https://doi.org/10.7717/peerj.581
Warnock JJ, Bobe G, Duesterdieck-Zellmer KF. Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs. PeerJ. 2014 Sep 30;2:e581. doi: 10.7717/peerj.581. eCollection 2014. PMID: 25289180; PMCID: PMC4183955.
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PeerJ. 2014 Sep 30;2:e581. doi: 10.7717/peerj.581. eCollection 2014.

Fibrochondrogenic potential of synoviocytes from osteoarthritic and normal joints cultured as tensioned bioscaffolds for meniscal tissue engineering in dogs.

PeerJ

Jennifer J Warnock, Gerd Bobe, Katja F Duesterdieck-Zellmer

Affiliations

  1. College of Veterinary Medicine, Oregon State University , Corvallis, OR , United States.
  2. Linus Pauling Institute, Oregon State University , Corvallis, OR , United States.

PMID: 25289180 PMCID: PMC4183955 DOI: 10.7717/peerj.581

Abstract

Meniscal tears are a common cause of stifle lameness in dogs. Use of autologous synoviocytes from the affected stifle is an attractive cell source for tissue engineering replacement fibrocartilage. However, the diseased state of these cells may impede in vitro fibrocartilage formation. Synoviocytes from 12 osteoarthritic ("oaTSB") and 6 normal joints ("nTSB") were cultured as tensioned bioscaffolds and compared for their ability to synthesize fibrocartilage sheets. Gene expression of collagens type I and II were higher and expression of interleukin-6 was lower in oaTSB versus nTSB. Compared with nTSB, oaTSB had more glycosaminoglycan and alpha smooth muscle staining and less collagen I and II staining on histologic analysis, whereas collagen and glycosaminoglycan quantities were similar. In conclusion, osteoarthritic joint-origin synoviocytes can produce extracellular matrix components of meniscal fibrocartilage at similar levels to normal joint-origin synoviocytes, which makes them a potential cell source for canine meniscal tissue engineering.

Keywords: Cell culture; Meniscus; Osteoarthritis; Synovium; Tissue engineering

References

  1. Clin Exp Immunol. 1997 Jan;107(1):112-9 - PubMed
  2. J Orthop Res. 2008 Jun;26(6):809-15 - PubMed
  3. J Orthop Res. 2001 Jul;19(4):659-64 - PubMed
  4. Vet Comp Orthop Traumatol. 2012;25(2):160-6 - PubMed
  5. J Surg Res. 1995 May;58(5):478-84 - PubMed
  6. Int J Tissue React. 1990;12(1):21-31 - PubMed
  7. Crit Rev Microbiol. 2008;34(1):1-12 - PubMed
  8. FASEB J. 2004 May;18(7):816-27 - PubMed
  9. Connect Tissue Res. 2005;46(2):83-91 - PubMed
  10. Tissue Eng Part A. 2008 Dec;14(12):2041-9 - PubMed
  11. Tissue Eng Part A. 2011 Mar;17(5-6):703-12 - PubMed
  12. Osteoarthritis Cartilage. 2009 Sep;17(9):1236-43 - PubMed
  13. Clin Orthop Relat Res. 1990 Mar;(252):8-18 - PubMed
  14. J Rheumatol. 1997 Feb;24(2):365-71 - PubMed
  15. J Appl Physiol (1985). 2011 Jun;110(6):1549-54 - PubMed
  16. Res Vet Sci. 2013 Dec;95(3):1201-9 - PubMed
  17. Tissue Eng. 2005 Jul-Aug;11(7-8):1141-8 - PubMed
  18. Clin Exp Rheumatol. 2002 Sep-Oct;20(5):633-40 - PubMed
  19. J Bone Joint Surg Am. 2000 Aug;82(8):1071-82 - PubMed
  20. J Trauma. 1991 Mar;31(3):342-50 - PubMed
  21. Lab Invest. 1990 Nov;63(5):647-56 - PubMed
  22. Am J Pathol. 2006 Aug;169(2):491-502 - PubMed
  23. Matrix Biol. 2003 Jun;22(4):363-72 - PubMed
  24. Connect Tissue Res. 1987;16(3):269-79 - PubMed
  25. Curr Protoc Immunol. 2001 May;Appendix 3:Appendix 3B - PubMed
  26. Tissue Eng. 2004 Jul-Aug;10(7-8):1214-23 - PubMed
  27. Nat Protoc. 2008;3(6):1101-8 - PubMed
  28. Tissue Eng Part A. 2010 Jul;16(7):2281-94 - PubMed
  29. Clin Orthop Relat Res. 1990 Mar;(252):19-31 - PubMed
  30. Wound Repair Regen. 2001 Jan-Feb;9(1):11-8 - PubMed
  31. Res Vet Sci. 2012 Dec;93(3):1472-80 - PubMed
  32. Clin Orthop Relat Res. 2008 Aug;466(8):1880-9 - PubMed
  33. J Periodontol. 2003 Mar;74(3):296-306 - PubMed
  34. Int J Mol Med. 2012 Nov;30(5):1119-25 - PubMed
  35. J Orthop Res. 1997 Mar;15(2):213-20 - PubMed
  36. FEBS Lett. 1983 Jul 25;158(2):265-70 - PubMed
  37. J Orthop Res. 2005 Sep;23(5):1184-90 - PubMed
  38. Am J Vet Res. 2011 Oct;72(10):1369-77 - PubMed
  39. Tissue Eng Part A. 2010 Jan;16(1):67-79 - PubMed
  40. Biochim Biophys Acta. 1986 Sep 4;883(2):173-7 - PubMed
  41. Biomaterials. 2007 Dec;28(36):5462-70 - PubMed
  42. Clin Biochem. 1996 Jun;29(3):225-9 - PubMed
  43. Differentiation. 2008 Dec;76(10):1044-56 - PubMed
  44. Wound Repair Regen. 2000 Nov-Dec;8(6):554-61 - PubMed
  45. Arthroscopy. 1997 Aug;13(4):517-21 - PubMed
  46. Ann Rheum Dis. 2005 Sep;64(9):1263-7 - PubMed
  47. Scand J Med Sci Sports. 2009 Aug;19(4):490-9 - PubMed
  48. Vet Surg. 2013 Feb;42(2):137-46 - PubMed
  49. J Physiol. 1996 Sep 15;495 ( Pt 3):803-20 - PubMed
  50. Prog Biophys Mol Biol. 2011 Sep;106(3):517-24 - PubMed
  51. Arthritis Rheum. 1976 May-Jun;19(3):581-92 - PubMed
  52. Am J Vet Res. 1998 Feb;59(2):213-6 - PubMed
  53. Cell Tissue Bank. 2013 Dec;14(4):699-706 - PubMed
  54. Clin Orthop Relat Res. 1975;(109):178-83 - PubMed
  55. Vet Surg. 2011 Jul;40(5):531-43 - PubMed
  56. J Orthop Res. 2012 Nov;30(11):1695-701 - PubMed
  57. J Orthop Res. 2005 Jan;23(1):142-9 - PubMed
  58. J Biomed Mater Res B Appl Biomater. 2006 Feb;76(2):389-96 - PubMed
  59. Am J Vet Res. 2011 Apr;72(4):500-11 - PubMed
  60. PLoS One. 2012;7(8):e43616 - PubMed
  61. J Orthop Sci. 2007 Nov;12(6):555-61 - PubMed
  62. Vet J. 2009 Jan;179(1):10-24 - PubMed
  63. Cytokine. 1990 Mar;2(2):149-55 - PubMed
  64. J Bone Joint Surg Br. 1961 Nov;43-B:752-7 - PubMed
  65. J Orthop Res. 2006 Nov;24(11):2087-94 - PubMed
  66. Rheumatology (Oxford). 2005 Feb;44(2):164-71 - PubMed
  67. Anal Biochem. 1987 Apr;162(1):156-9 - PubMed

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